Detailed information of TA system TA085172

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2868097..2868806
• Replicon: chromosome
• Accession: NZ_CP023630
• Organism: Mycobacterium tuberculosis strain MDRMA2441

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CP486_RS13955
• Protein ID: WP_031648662.1
• Coordinates: 2868097..2868510 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P95006
• Locus tag: CP486_RS13960
• Protein ID: WP_003413167.1
• Coordinates: 2868549..2868806 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP486_RS13920	2863150..2864355	-	1206	WP_003413027.1	chorismate synthase	-
CP486_RS13925	2864463..2864777	+	315	WP_009937839.1	hypothetical protein	-
CP486_RS13930	2865073..2866284	+	1212	WP_003900845.1	alpha/beta hydrolase family protein	-
CP486_RS13940	2866411..2867070	+	660	WP_003900846.1	LppA family lipoprotein	-
CP486_RS13945	2867067..2867729	+	663	WP_003900848.1	hypothetical protein	-
CP486_RS13950	2867726..2868004	+	279	WP_003901422.1	type II toxin-antitoxin system VapB family antitoxin	-
CP486_RS13955	2868097..2868510	+	414	WP_031648662.1	PIN domain nuclease	Toxin
CP486_RS13960	2868549..2868806	+	258	WP_003413167.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP486_RS13965	2868803..2869180	+	378	WP_003413174.1	type II toxin-antitoxin system VapC family toxin	-
CP486_RS13970	2869196..2869570	-	375	WP_003413177.1	hypothetical protein	-
CP486_RS13975	2869670..2870065	-	396	WP_003413180.1	type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20	-
CP486_RS13980	2870062..2870307	-	246	WP_003413183.1	type II toxin-antitoxin system antitoxin VapB20	-
CP486_RS13990	2870718..2871137	-	420	WP_003413190.1	A24 family peptidase	-
CP486_RS13995	2871149..2871958	-	810	WP_003413193.1	shikimate dehydrogenase	-
CP486_RS14000	2871955..2873208	-	1254	WP_003413196.1	endolytic transglycosylase MltG	-
CP486_RS14005	2873201..2873713	-	513	WP_003413197.1	Holliday junction resolvase RuvX	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (4-122)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14915.84 Da
• Isoelectric Point: 5.4673

>T85172 WP_031648662.1 NZ_CP023630:2868097-2868510 [Mycobacterium tuberculosis]
MVFCVDTSAWHHAARPEVARRWLAALSADQIGICDHVRLEILYSANSATDYDALADELDGLARIPVGAETFTRACQVQRE
LAHVAGLHHRSVKIADLVIAAAAELSGTIVWHYDENYDRVAAITGQPTEGIVPRGTL

Nucleotide

• Length: 414 bp

>T85172 NZ_CP023630:2868097-2868510 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGGGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9453.63 Da
• Isoelectric Point: 10.6555

>AT85172 WP_003413167.1 NZ_CP023630:2868549-2868806 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Nucleotide

• Length: 258 bp

>AT85172 NZ_CP023630:2868549-2868806 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A829C9W5