Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 2234260..2234846 Replicon chromosome
Accession NZ_CP023630
Organism Mycobacterium tuberculosis strain MDRMA2441

Toxin (Protein)


Gene name mazF Uniprot ID G0TLY3
Locus tag CP486_RS10855 Protein ID WP_003410010.1
Coordinates 2234260..2234604 (-) Length 115 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL58
Locus tag CP486_RS10860 Protein ID WP_003410014.1
Coordinates 2234598..2234846 (-) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP486_RS10815 2229966..2230565 + 600 WP_003409989.1 L-lysine exporter -
CP486_RS10820 2230981..2231409 + 429 WP_003409992.1 cellulose-binding protein -
CP486_RS10825 2231635..2232174 + 540 WP_003900446.1 23S rRNA (adenine(2058)-N(6))-methyltransferase Erm(37) -
CP486_RS10835 2232694..2233254 - 561 WP_003410001.1 RES family NAD+ phosphorylase -
CP486_RS10840 2233251..2233592 - 342 WP_003410003.1 DUF2384 domain-containing protein -
CP486_RS10845 2233678..2233935 + 258 WP_003410006.1 hypothetical protein -
CP486_RS10850 2233836..2234171 - 336 WP_003410009.1 dehydrogenase -
CP486_RS10855 2234260..2234604 - 345 WP_003410010.1 type II toxin-antitoxin system toxin endoribonuclease MazF6 Toxin
CP486_RS10860 2234598..2234846 - 249 WP_003410014.1 ribbon-helix-helix protein, CopG family Antitoxin
CP486_RS10865 2234946..2237261 - 2316 WP_003899120.1 cation transporter ATPase CptG -
CP486_RS10870 2237258..2237530 - 273 WP_003410017.1 DUF1490 family protein -
CP486_RS10875 2237583..2237939 - 357 WP_003410018.1 Cd(II)/Pb(II)-sensing metalloregulatory transcriptional regulator CmtR -
CP486_RS10880 2238096..2238863 + 768 WP_003410019.1 hemerythrin domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(5-112)

Antitoxin

Ribbon-helix-helix protein, CopG

(5-38)


Sequences

Toxin        


Download         Length: 115 a.a.        Molecular weight: 12230.12 Da        Isoelectric Point: 9.8887

>T85164 WP_003410010.1 NZ_CP023630:c2234604-2234260 [Mycobacterium tuberculosis]
VVISRAEIYWADLGPPSGSQPAKRRPVLVIQSDPYNASRLATVIAAVITSNTALAAMPGNVFLPATTTRLPRDSVVNVTA
IVTLNKTDLTDRVGEVPASLMHEVDRGLRRVLDL

Download         Length: 345 bp

>T85164 NZ_CP023630:c2234604-2234260 [Mycobacterium tuberculosis]
GTGGTGATTAGTCGTGCCGAGATCTACTGGGCTGACCTCGGGCCGCCATCAGGCAGTCAGCCGGCGAAGCGCCGCCCGGT
GCTCGTAATCCAGTCAGATCCGTACAACGCAAGTCGCCTTGCCACTGTGATCGCAGCGGTGATCACGTCCAATACGGCGC
TGGCGGCAATGCCCGGCAACGTGTTCTTGCCCGCGACCACAACGCGACTGCCACGTGACTCGGTCGTCAACGTCACGGCG
ATTGTCACGCTCAACAAGACTGACCTCACCGACCGAGTTGGGGAGGTGCCAGCGAGCTTGATGCACGAGGTTGACCGAGG
ACTTCGTCGCGTACTGGACCTTTGA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 9290.34 Da        Isoelectric Point: 4.3757

>AT85164 WP_003410014.1 NZ_CP023630:c2234846-2234598 [Mycobacterium tuberculosis]
MKTAISLPDETFDRVSRRASELGMSRSEFFTKAAQRYLHELDAQLLTGQIDRALESIHGTDEAEALAVANAYRVLETMDD
EW

Download         Length: 249 bp

>AT85164 NZ_CP023630:c2234846-2234598 [Mycobacterium tuberculosis]
ATGAAGACAGCTATTTCTCTGCCGGATGAGACGTTCGATCGGGTATCGCGGCGTGCGAGTGAGCTCGGCATGAGTCGGTC
CGAGTTCTTCACGAAGGCTGCGCAGCGCTACCTGCACGAGCTGGACGCCCAATTGCTCACGGGCCAGATCGACAGGGCTC
TAGAGAGCATCCATGGCACCGACGAAGCGGAGGCCCTCGCCGTGGCCAACGCATACCGCGTGCTAGAAACCATGGACGAT
GAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5HK3
PDB 5HK0
PDB 5HKC

Antitoxin

Source ID Structure
PDB 7WJ0
PDB 7WNR
AlphaFold DB A0A7U4FAW9

References