Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1946983..1947596 Replicon chromosome
Accession NZ_CP023630
Organism Mycobacterium tuberculosis strain MDRMA2441

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag CP486_RS09385 Protein ID WP_003408465.1
Coordinates 1946983..1947372 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag CP486_RS09390 Protein ID WP_003408469.1
Coordinates 1947369..1947596 (-) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP486_RS09355 1942612..1943526 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
CP486_RS09360 1943529..1944359 + 831 WP_003916363.1 cyclase family protein -
CP486_RS09365 1944359..1944709 + 351 WP_003898986.1 cupin domain-containing protein -
CP486_RS09370 1944762..1945580 + 819 WP_003408456.1 3-keto-5-aminohexanoate cleavage protein -
CP486_RS09375 1945594..1946373 + 780 WP_003408460.1 IclR family transcriptional regulator -
CP486_RS09385 1946983..1947372 - 390 WP_003408465.1 PIN domain-containing protein Toxin
CP486_RS09390 1947369..1947596 - 228 WP_003408469.1 antitoxin Antitoxin
CP486_RS09395 1947814..1949298 + 1485 WP_003408473.1 carboxylase -
CP486_RS09400 1949295..1950542 + 1248 WP_003408476.1 serine hydrolase -
CP486_RS09405 1950585..1951004 - 420 WP_003408483.1 hypothetical protein -
CP486_RS09410 1950994..1951704 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-116)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T85157 WP_003408465.1 NZ_CP023630:c1947372-1946983 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T85157 NZ_CP023630:c1947372-1946983 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT85157 WP_003408469.1 NZ_CP023630:c1947596-1947369 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT85157 NZ_CP023630:c1947596-1947369 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9

Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References