Detailed information of TA system TA085146

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): unclassified/Polyketide_cyc2(toxin)
• Location: 1014614..1015233
• Replicon: chromosome
• Accession: NZ_CP023630
• Organism: Mycobacterium tuberculosis strain MDRMA2441

Toxin (Protein)

• Gene name: novel[antitoxin]
• Uniprot ID: -
• Locus tag: CP486_RS04955
• Protein ID: WP_003404726.1
• Coordinates: 1014799..1015233 (+)
• Length: 145 a.a.

Antitoxin (Protein)

• Gene name: novel[toxin]
• Uniprot ID: P9WJ06
• Locus tag: CP486_RS04950
• Protein ID: WP_003898641.1
• Coordinates: 1014614..1014793 (+)
• Length: 60 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP486_RS04940	1010087..1011667	+	1581	WP_003909313.1	serine hydrolase	-
CP486_RS04945	1011664..1014057	+	2394	WP_003898639.1	cation-translocating P-type ATPase	-
CP486_RS21710	1014330..1014488	+	159	WP_003404720.1	hypothetical protein	-
CP486_RS04950	1014614..1014793	+	180	WP_003898641.1	antitoxin	Antitoxin
CP486_RS04955	1014799..1015233	+	435	WP_003404726.1	SRPBCC family protein	Toxin
CP486_RS04960	1015331..1016104	+	774	WP_003404735.1	VOC family protein	-
CP486_RS04965	1016169..1016618	+	450	WP_003404738.1	hypothetical protein	-
CP486_RS04970	1016753..1016983	-	231	WP_003898642.1	hypothetical protein	-
CP486_RS04975	1017150..1018658	-	1509	WP_003404742.1	carotenoid oxygenase family protein	-
CP486_RS04980	1018660..1019898	-	1239	WP_003404744.1	acetyl-CoA acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Polyketide cyclase/dehydrase (1-142)

Antitoxin

• Antitoxin Rv0909/MT0933 (4-50)

Sequences

Toxin

Protein

• Length: 145 a.a.
• Molecular weight: 15754.47 Da
• Isoelectric Point: 9.7977

>T85146 WP_003404726.1 NZ_CP023630:1014799-1015233 [Mycobacterium tuberculosis]
MAKLSGSIDVPLPPEEAWMHASDLTRYREWLTIHKVWRSKLPEVLEKGTVVESYVEVKGMPNRIKWTIVRYKPPEGMTLN
GDGVGGVKVKLIAKVAPKEHGSVVSFDVHLGGPALLGPIGMIVAAALRADIRESLQNFVTVFAG

Nucleotide

• Length: 435 bp

>T85146 NZ_CP023630:1014799-1015233 [Mycobacterium tuberculosis]
ATGGCGAAACTGTCCGGATCCATCGACGTACCGCTGCCACCGGAGGAAGCCTGGATGCACGCCTCCGATCTGACTCGTTA
CCGAGAGTGGCTGACCATCCACAAGGTATGGCGCAGCAAGTTGCCCGAAGTGCTCGAGAAGGGCACGGTCGTCGAGTCGT
ATGTCGAGGTCAAGGGCATGCCCAACCGGATCAAGTGGACGATCGTGCGGTACAAACCCCCGGAGGGCATGACGCTCAAC
GGCGACGGTGTGGGTGGTGTCAAAGTCAAGCTGATCGCTAAGGTAGCGCCGAAAGAGCACGGCTCCGTCGTCAGCTTCGA
TGTGCACCTCGGCGGCCCGGCCCTGCTCGGGCCGATCGGCATGATCGTCGCCGCTGCATTGCGAGCCGACATCCGCGAAT
CGCTGCAGAACTTCGTCACGGTGTTTGCCGGCTGA

Antitoxin

Protein

• Length: 60 a.a.
• Molecular weight: 6403.07 Da
• Isoelectric Point: 4.5393

>AT85146 WP_003898641.1 NZ_CP023630:1014614-1014793 [Mycobacterium tuberculosis]
MGILDKVKNLLSQNADKVETVINKAGEFVDEQTQGNYSDAIHKLHDAASNVVGMSDQQS

Nucleotide

• Length: 180 bp

>AT85146 NZ_CP023630:1014614-1014793 [Mycobacterium tuberculosis]
ATGGGAATCCTGGACAAGGTAAAGAACCTGCTGTCGCAGAACGCCGACAAGGTCGAGACGGTGATCAACAAAGCGGGCGA
ATTCGTCGACGAGCAGACGCAAGGCAATTATTCTGACGCCATCCACAAGCTGCATGACGCGGCCAGCAACGTCGTCGGCA
TGAGCGACCAGCAGAGCTAG

Structures

Antitoxin

• AlphaFold DB: A0A045KDK5