Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapC-mazE/PIN(toxin)
Location 754923..755715 Replicon chromosome
Accession NZ_CP023630
Organism Mycobacterium tuberculosis strain MDRMA2441

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB2
Locus tag CP486_RS03565 Protein ID WP_003403386.1
Coordinates 755278..755715 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag CP486_RS03560 Protein ID WP_003403381.1
Coordinates 754923..755168 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP486_RS03530 749943..751448 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
CP486_RS03535 751529..752539 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
CP486_RS03540 752927..753310 - 384 WP_003403365.1 ribonuclease VapC6 -
CP486_RS03545 753405..753560 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
CP486_RS03550 753636..754352 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
CP486_RS03555 754628..754936 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
CP486_RS03560 754923..755168 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
CP486_RS03565 755278..755715 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin Toxin
CP486_RS03570 755712..755966 - 255 WP_003911263.1 antitoxin VapB7 -
CP486_RS03575 756080..758443 + 2364 WP_003898547.1 arylsulfatase AtsD -
CP486_RS03580 758506..758832 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CP486_RS03585 758744..759082 + 339 WP_003403405.1 PIN domain-containing protein -
CP486_RS03590 759079..759252 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T85143 WP_003403386.1 NZ_CP023630:c755715-755278 [Mycobacterium tuberculosis]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T85143 NZ_CP023630:c755715-755278 [Mycobacterium tuberculosis]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT85143 WP_003403381.1 NZ_CP023630:c755168-754923 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT85143 NZ_CP023630:c755168-754923 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSW5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References