Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 752927..753560 Replicon chromosome
Accession NZ_CP023630
Organism Mycobacterium tuberculosis strain MDRMA2441

Toxin (Protein)


Gene name vapC Uniprot ID G0TQD6
Locus tag CP486_RS03540 Protein ID WP_003403365.1
Coordinates 752927..753310 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ56
Locus tag CP486_RS03545 Protein ID WP_003403368.1
Coordinates 753405..753560 (-) Length 52 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP486_RS03515 748219..748755 + 537 WP_003403341.1 50S ribosomal protein L10 -
CP486_RS03520 748792..749184 + 393 WP_003403353.1 50S ribosomal protein L7/L12 -
CP486_RS03525 749177..749872 - 696 WP_023637352.1 TetR/AcrR family transcriptional regulator -
CP486_RS03530 749943..751448 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
CP486_RS03535 751529..752539 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
CP486_RS03540 752927..753310 - 384 WP_003403365.1 ribonuclease VapC6 Toxin
CP486_RS03545 753405..753560 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP486_RS03550 753636..754352 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
CP486_RS03555 754628..754936 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
CP486_RS03560 754923..755168 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family -
CP486_RS03565 755278..755715 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
CP486_RS03570 755712..755966 - 255 WP_003911263.1 antitoxin VapB7 -
CP486_RS03575 756080..758443 + 2364 WP_003898547.1 arylsulfatase AtsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13959.73 Da        Isoelectric Point: 6.0803

>T85141 WP_003403365.1 NZ_CP023630:c753310-752927 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Download         Length: 384 bp

>T85141 NZ_CP023630:c753310-752927 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 52 a.a.        Molecular weight: 5872.72 Da        Isoelectric Point: 5.7734

>AT85141 WP_003403368.1 NZ_CP023630:c753560-753405 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Download         Length: 156 bp

>AT85141 NZ_CP023630:c753560-753405 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQD6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSF8

References