Detailed information of TA system TA085136

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/AbrB(antitoxin)
• Location: 697098..697744
• Replicon: chromosome
• Accession: NZ_CP023630
• Organism: Mycobacterium tuberculosis strain MDRMA2441

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQ88
• Locus tag: CP486_RS03200
• Protein ID: WP_003403137.1
• Coordinates: 697098..697511 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TQ89
• Locus tag: CP486_RS03205
• Protein ID: WP_003403139.1
• Coordinates: 697508..697744 (-)
• Length: 79 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP486_RS03180	693181..694731	+	1551	WP_031648549.1	MCE family protein	-
CP486_RS03185	694783..695175	-	393	WP_003403122.1	type II toxin-antitoxin system toxin ribonuclease C4	-
CP486_RS03190	695172..695429	-	258	WP_003403125.1	type II toxin-antitoxin system antitoxin VapB4	-
CP486_RS03195	695612..696847	-	1236	WP_003403128.1	ATP-binding protein	-
CP486_RS03200	697098..697511	-	414	WP_003403137.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP486_RS03205	697508..697744	-	237	WP_003403139.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
CP486_RS03210	697848..698354	-	507	WP_003900973.1	two component system sensor kinase HK1	-
CP486_RS03215	698468..698998	-	531	WP_003917320.1	two component system sensor kinase HK2	-
CP486_RS03220	698982..699677	-	696	WP_003918099.1	two-component system response regulator TcrA	-
CP486_RS03225	699800..700111	+	312	WP_003403164.1	hypothetical protein	-
CP486_RS03230	700183..701133	+	951	WP_003900184.1	DUF1259 domain-containing protein	-
CP486_RS03235	701374..701958	+	585	WP_003403171.1	IS607-like element IS1536 family transposase	-
CP486_RS03240	701960..702670	+	711	Protein_626	transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-126)

Antitoxin

• SpoVT-AbrB domain (3-43)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14786.14 Da
• Isoelectric Point: 8.1405

>T85136 WP_003403137.1 NZ_CP023630:c697511-697098 [Mycobacterium tuberculosis]
VKPPLAVDTSVAIPLLVRTHTAHAAVVAWWAHREAALCGHALAETYSVLTRLPRDLRLAPMDAARLLTERFAAPLLLSSR
TTEHLPRVLAQFEITGGAVYDALVALAAAEHRAELATRDARAKDTYEKIGVHVVVAA

Nucleotide

• Length: 414 bp

>T85136 NZ_CP023630:c697511-697098 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 79 a.a.
• Molecular weight: 8212.41 Da
• Isoelectric Point: 5.0351

>AT85136 WP_003403139.1 NZ_CP023630:c697744-697508 [Mycobacterium tuberculosis]
MKAVVDAAGRIVVPKPLREALGLQPGSTVEISRYGAGLHLIPTGRTARLEEENGVLVATGETTIDDEVVFGLIDSGRK

Nucleotide

• Length: 237 bp

>AT85136 NZ_CP023630:c697744-697508 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA

Structures

Toxin

• AlphaFold DB: G0TQ88

Antitoxin

• AlphaFold DB: G0TQ89