Detailed information of TA system TA085133

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN-CcdA
• Location: 640172..640848
• Replicon: chromosome
• Accession: NZ_CP023630
• Organism: Mycobacterium tuberculosis strain MDRMA2441

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TPQ9
• Locus tag: CP486_RS02940
• Protein ID: WP_003898500.1
• Coordinates: 640172..640585 (-)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TPR0
• Locus tag: CP486_RS02945
• Protein ID: WP_003402915.1
• Coordinates: 640582..640848 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP486_RS02910	635517..635819	-	303	WP_003402896.1	DUF3349 domain-containing protein	-
CP486_RS02915	635879..636157	-	279	WP_003402901.1	hypothetical protein	-
CP486_RS02920	636154..637407	-	1254	WP_003911224.1	inorganic phosphate transporter family protein	-
CP486_RS02925	637527..637913	-	387	WP_003402907.1	VOC family protein	-
CP486_RS02930	637976..638860	-	885	WP_003402909.1	SDR family oxidoreductase	-
CP486_RS02935	638956..639858	-	903	WP_003915439.1	1,4-dihydroxy-2-naphthoyl-CoA synthase	-
CP486_RS02940	640172..640585	-	414	WP_003898500.1	PIN domain-containing protein	Toxin
CP486_RS02945	640582..640848	-	267	WP_003402915.1	antitoxin VapB3	Antitoxin
CP486_RS02950	641040..642755	-	1716	WP_003402917.1	fatty-acid--CoA ligase FadD8	-
CP486_RS02955	642833..644437	+	1605	WP_003402920.1	amidohydrolase	-
CP486_RS02960	644434..645414	+	981	WP_003402923.1	o-succinylbenzoate synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Pae0151-like, PIN domain (13-129)

Antitoxin

• Post-segregation antitoxin CcdA (19-61)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 14667.78 Da
• Isoelectric Point: 7.3603

>T85133 WP_003898500.1 NZ_CP023630:c640585-640172 [Mycobacterium tuberculosis]
VRASPTSPPEQVVVDASAMVDLLARTSDRCSAVRARLARTAMHAPAHFDAEVLSALGRMQRAGALTVAYVDAALEELRQV
PVTRHGLSSLLAGAWSRRDTLRLTDALYVELAETAGLVLLTTDERLARAWPSAHAIG

Nucleotide

• Length: 414 bp

>T85133 NZ_CP023630:c640585-640172 [Mycobacterium tuberculosis]
GTGAGAGCATCGCCCACTTCGCCGCCGGAGCAGGTGGTCGTCGACGCGAGTGCCATGGTGGATCTACTGGCTCGCACTAG
CGATCGGTGCTCTGCGGTGCGCGCGCGGCTGGCTCGGACCGCGATGCACGCGCCGGCGCACTTCGATGCAGAGGTGTTGT
CGGCGCTGGGGCGCATGCAGCGCGCCGGCGCACTCACCGTTGCCTATGTCGATGCGGCACTGGAGGAGTTGCGACAGGTG
CCGGTGACTCGACACGGTCTTTCGTCGCTGCTTGCTGGAGCGTGGTCGCGCCGCGACACCCTCCGCCTGACCGATGCCCT
CTACGTCGAGCTGGCCGAAACGGCAGGTCTGGTGTTGTTGACCACCGACGAAAGATTGGCACGCGCCTGGCCCTCGGCTC
ACGCCATCGGCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9543.79 Da
• Isoelectric Point: 4.5994

>AT85133 WP_003402915.1 NZ_CP023630:c640848-640582 [Mycobacterium tuberculosis]
MLSRRTKTIVVCTLVCMARLNVYVPDELAERARARGLNVSALTQAAISAELENSATDAWLEGLEPRSTGARHDDVLGAID
AARDEFEA

Nucleotide

• Length: 267 bp

>AT85133 NZ_CP023630:c640848-640582 [Mycobacterium tuberculosis]
TTGTTGAGCCGGCGCACGAAAACGATCGTTGTGTGTACATTGGTGTGTATGGCTCGGTTGAACGTGTATGTGCCCGACGA
ATTGGCGGAGCGCGCCAGGGCGCGGGGCTTGAACGTCTCGGCGCTGACTCAGGCCGCGATCAGTGCCGAGTTGGAGAACT
CCGCAACCGATGCGTGGCTTGAGGGGTTGGAACCCAGAAGCACCGGCGCTCGGCATGATGACGTGCTGGGTGCGATCGAT
GCCGCTCGCGATGAGTTCGAAGCGTGA

Structures

Toxin

• AlphaFold DB: G0TPQ9

Antitoxin

• AlphaFold DB: G0TPR0