Detailed information of TA system TA085106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/PemK(toxin)
• Location: 2320707..2321343
• Replicon: chromosome
• Accession: NZ_CP023629
• Organism: Mycobacterium tuberculosis strain MDRMA2260

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CP485_RS11245
• Protein ID: WP_096875440.1
• Coordinates: 2320933..2321343 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: P9WJ84
• Locus tag: CP485_RS11240
• Protein ID: WP_003410651.1
• Coordinates: 2320707..2320940 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP485_RS11230	2316557..2316961	-	405	WP_003410645.1	PPOX class F420-dependent oxidoreductase	-
CP485_RS11235	2317045..2320629	-	3585	WP_003410648.1	cobaltochelatase subunit CobN	-
CP485_RS11240	2320707..2320940	+	234	WP_003410651.1	type II toxin-antitoxin system antitoxin MazE7	Antitoxin
CP485_RS11245	2320933..2321343	+	411	WP_096875440.1	type II toxin-antitoxin system toxin endoribonuclease MazF7	Toxin
CP485_RS11250	2321327..2322418	+	1092	WP_003900454.1	precorrin-3B synthase	-
CP485_RS11255	2322428..2323054	+	627	WP_003410658.1	precorrin-8X methylmutase	-
CP485_RS11260	2323051..2324577	+	1527	WP_003410659.1	precorrin-2 C(20)-methyltransferase	-
CP485_RS11265	2324523..2325746	-	1224	WP_003901321.1	class I SAM-dependent methyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (5-110)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.4815

>T85106 WP_096875440.1 NZ_CP023629:2320933-2321343 [Mycobacterium tuberculosis]
MAEPRRGDLWLVSLGAARAGEPGKHRPAVVVSVDELLTGIDDELVVVVPVSSSRSRTPLRPPVAPSEGVAADSVAVCRDV
RAVARARLVERLGALKPATMRAIENALTLILGLPTGPERGEAATHSPVRWTGGRDP

Nucleotide

• Length: 411 bp

>T85106 NZ_CP023629:2320933-2321343 [Mycobacterium tuberculosis]
TTGGCTGAGCCACGGCGAGGAGACCTTTGGCTGGTCAGCCTCGGCGCCGCTCGCGCGGGTGAGCCCGGCAAGCATCGGCC
CGCGGTGGTCGTTTCCGTGGACGAGCTACTCACCGGAATCGACGACGAACTCGTTGTCGTCGTGCCGGTGTCAAGCTCGC
GCTCCCGCACCCCACTCCGGCCACCTGTCGCGCCCTCAGAAGGTGTAGCTGCCGATAGCGTCGCGGTGTGCCGCGACGTC
CGCGCGGTCGCTCGTGCCCGACTCGTGGAGCGACTCGGCGCCCTCAAACCCGCCACGATGCGCGCAATCGAAAACGCCCT
GACCCTGATCCTCGGCCTCCCGACGGGACCTGAGCGCGGCGAGGCGGCGACCCATTCTCCCGTACGGTGGACGGGTGGCC
GGGACCCGTGA

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8450.25 Da
• Isoelectric Point: 4.7954

>AT85106 WP_003410651.1 NZ_CP023629:2320707-2320940 [Mycobacterium tuberculosis]
MSTSTTIRVSTQTRDRLAAQARERGISMSALLTELAAQAERQAIFRAEREASHAETTTQAVRDEDREWEGTVGDGLG

Nucleotide

• Length: 234 bp

>AT85106 NZ_CP023629:2320707-2320940 [Mycobacterium tuberculosis]
ATGTCTACATCCACGACGATTAGGGTTTCAACCCAGACTCGGGATCGTCTGGCCGCCCAAGCCCGCGAACGGGGAATCTC
GATGTCGGCTCTGCTCACCGAACTGGCCGCCCAGGCCGAGCGCCAGGCAATCTTCCGCGCCGAACGCGAGGCCTCGCACG
CCGAGACGACCACCCAGGCAGTCCGCGACGAGGACCGCGAGTGGGAGGGCACGGTAGGCGACGGCCTTGGCTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BV50