Detailed information of TA system TA085098

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194517..2195220
• Replicon: chromosome
• Accession: NZ_CP023629
• Organism: Mycobacterium tuberculosis strain MDRMA2260

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: G0TLK7
• Locus tag: CP485_RS10560
• Protein ID: WP_003409778.1
• Coordinates: 2194517..2194846 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: CP485_RS10565
• Protein ID: WP_003409780.1
• Coordinates: 2194843..2195220 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP485_RS10540	2190900..2191970	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
CP485_RS10545	2191967..2192482	+	516	WP_003409718.1	flavin reductase family protein	-
CP485_RS10550	2192479..2193540	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
CP485_RS10555	2193537..2194307	+	771	WP_003409775.1	SDR family oxidoreductase	-
CP485_RS10560	2194517..2194846	-	330	WP_003409778.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CP485_RS10565	2194843..2195220	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
CP485_RS10570	2195217..2195807	-	591	WP_003409784.1	SEC-C domain-containing protein	-
CP485_RS10575	2195862..2197226	+	1365	WP_003903691.1	HNH endonuclease	-
CP485_RS10580	2197381..2197833	-	453	WP_003899095.1	lipoprotein	-
CP485_RS10585	2197897..2198298	+	402	WP_003409869.1	hypothetical protein	-
CP485_RS10590	2198291..2198473	-	183	WP_003409870.1	hypothetical protein	-
CP485_RS10595	2198587..2198937	-	351	WP_003899096.1	hypothetical protein	-
CP485_RS10600	2198948..2199850	-	903	WP_003899097.1	hypothetical protein	-
CP485_RS10605	2199871..2200062	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (7-103)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11787.82 Da
• Isoelectric Point: 8.5361

>T85098 WP_003409778.1 NZ_CP023629:c2194846-2194517 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLVNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T85098 NZ_CP023629:c2194846-2194517 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT85098 WP_003409780.1 NZ_CP023629:c2195220-2194843 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT85098 NZ_CP023629:c2195220-2194843 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: G0TLK7

Antitoxin

• AlphaFold DB: G0TLK8