Detailed information of TA system TA085063

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3707591..3708265
• Replicon: chromosome
• Accession: NZ_CP023628
• Organism: Mycobacterium tuberculosis strain MDRMA2082

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF52
• Locus tag: CP484_RS18170
• Protein ID: WP_003417282.1
• Coordinates: 3707591..3708019 (-)
• Length: 143 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TI59
• Locus tag: CP484_RS18175
• Protein ID: WP_003417286.1
• Coordinates: 3708023..3708265 (-)
• Length: 81 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP484_RS18145	3703413..3703814	-	402	WP_003417264.1	cytidine deaminase	-
CP484_RS18150	3703955..3704389	+	435	WP_003900017.1	succinate dehydrogenase, cytochrome b556 subunit	-
CP484_RS18155	3704386..3704820	+	435	WP_003417269.1	succinate dehydrogenase hydrophobic membrane anchor subunit	-
CP484_RS18160	3704949..3706721	+	1773	WP_003417273.1	succinate dehydrogenase flavoprotein subunit	-
CP484_RS18165	3706721..3707512	+	792	WP_003417279.1	succinate dehydrogenase iron-sulfur subunit	-
CP484_RS18170	3707591..3708019	-	429	WP_003417282.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP484_RS18175	3708023..3708265	-	243	WP_003417286.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP484_RS18180	3708387..3709001	-	615	WP_003417288.1	class I SAM-dependent methyltransferase	-
CP484_RS18185	3708998..3709663	-	666	WP_003417290.1	molybdopterin converting factor subunit 1	-
CP484_RS18190	3709664..3710197	-	534	WP_003417293.1	cyclic pyranopterin monophosphate synthase MoaC	-
CP484_RS18195	3710194..3710283	-	90	WP_009934953.1	pterin-4-alpha-carbinolamine dehydratase moaB3	-
CP484_RS18200	3710383..3711644	+	1262	WP_087902221.1	IS3-like element IS987 family transposase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-141)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 143 a.a.
• Molecular weight: 15834.16 Da
• Isoelectric Point: 8.5471

>T85063 WP_003417282.1 NZ_CP023628:c3708019-3707591 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Nucleotide

• Length: 429 bp

>T85063 NZ_CP023628:c3708019-3707591 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 81 a.a.
• Molecular weight: 8849.86 Da
• Isoelectric Point: 5.1007

>AT85063 WP_003417286.1 NZ_CP023628:c3708265-3708023 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Nucleotide

• Length: 243 bp

>AT85063 NZ_CP023628:c3708265-3708023 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBL0

Antitoxin

• AlphaFold DB: G0TI59