Detailed information of TA system TA085034

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 1764756..1765369
• Replicon: chromosome
• Accession: NZ_CP023628
• Organism: Mycobacterium tuberculosis strain MDRMA2082

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P64880
• Locus tag: CP484_RS08535
• Protein ID: WP_003407786.1
• Coordinates: 1764965..1765369 (+)
• Length: 135 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CP484_RS08530
• Protein ID: WP_198403690.1
• Coordinates: 1764756..1764959 (+)
• Length: 68 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP484_RS08500	1760161..1760541	+	381	WP_003407768.1	fumarate reductase subunit C	-
CP484_RS08505	1760538..1760915	+	378	WP_003407771.1	fumarate reductase subunit FrdD	-
CP484_RS08510	1760983..1761591	+	609	WP_003407773.1	TetR/AcrR family transcriptional regulator	-
CP484_RS08515	1761775..1762923	+	1149	Protein_1610	MMPL family transporter	-
CP484_RS08520	1762933..1763379	+	447	WP_003407780.1	F420H(2)-dependent quinone reductase	-
CP484_RS08525	1763414..1764703	+	1290	WP_003407781.1	threonine ammonia-lyase	-
CP484_RS08530	1764756..1764959	+	204	WP_198403690.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP484_RS08535	1764965..1765369	+	405	WP_003407786.1	type II toxin-antitoxin system toxin ribonuclease VapC11	Toxin
CP484_RS08540	1765386..1767128	-	1743	WP_003407788.1	malto-oligosyltrehalose trehalohydrolase	-
CP484_RS08545	1767121..1769418	-	2298	WP_003407790.1	malto-oligosyltrehalose synthase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (2-44)

Sequences

Toxin

Protein

• Length: 135 a.a.
• Molecular weight: 14650.76 Da
• Isoelectric Point: 5.6587

>T85034 WP_003407786.1 NZ_CP023628:1764965-1765369 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Nucleotide

• Length: 405 bp

>T85034 NZ_CP023628:1764965-1765369 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 68 a.a.
• Molecular weight: 7547.56 Da
• Isoelectric Point: 4.3950

>AT85034 WP_198403690.1 NZ_CP023628:1764756-1764959 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWECDLDDLRSDRPD

Nucleotide

• Length: 204 bp

>AT85034 NZ_CP023628:1764756-1764959 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAATGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• PDB: 6A7V