Detailed information of TA system TA085005

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-PHD
• Location: 3825970..3826655
• Replicon: chromosome
• Accession: NZ_CP023627
• Organism: Mycobacterium tuberculosis strain MDRMA2019

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TIR9
• Locus tag: CP483_RS18645
• Protein ID: WP_003417998.1
• Coordinates: 3826245..3826655 (+)
• Length: 137 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CP483_RS18640
• Protein ID: WP_003912220.1
• Coordinates: 3825970..3826248 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP483_RS18620	3821959..3823560	-	1602	WP_003417969.1	FAD/NAD(P)-binding protein	-
CP483_RS18625	3823577..3824281	-	705	WP_003417980.1	dTDP-4-amino-4,6-dideoxyglucose formyltransferase	-
CP483_RS18630	3824399..3824965	-	567	WP_003417984.1	TetR/AcrR family transcriptional regulator	-
CP483_RS18635	3825027..3825914	+	888	WP_003900050.1	alpha-ketoglutarate-dependent sulfate ester dioxygenase	-
CP483_RS18640	3825970..3826248	+	279	WP_003912220.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CP483_RS18645	3826245..3826655	+	411	WP_003417998.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP483_RS18650	3826688..3828424	-	1737	WP_003418002.1	cholesterol oxidase	-
CP483_RS18655	3828480..3829607	-	1128	WP_003418005.1	GuaB3 family IMP dehydrogenase-related protein	-
CP483_RS18660	3829627..3831216	-	1590	WP_003900682.1	IMP dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-118)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (3-54)

Sequences

Toxin

Protein

• Length: 137 a.a.
• Molecular weight: 14695.84 Da
• Isoelectric Point: 5.1784

>T85005 WP_003417998.1 NZ_CP023627:3826245-3826655 [Mycobacterium tuberculosis]
VIYMDTSALTKLLISEPETTELRTWLTAQSGQGEDAATSTLGRVELMRVVARYGQPGQTERARYLLDGLDILPLTEPVIG
LAETIGPATLRSLDAIHLAAAAQIKRELTAFVTYDHRLLSGCREVGFVTASPGAVR

Nucleotide

• Length: 411 bp

>T85005 NZ_CP023627:3826245-3826655 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10280.75 Da
• Isoelectric Point: 10.1819

>AT85005 WP_003912220.1 NZ_CP023627:3825970-3826248 [Mycobacterium tuberculosis]
VEAIGIRELRQHASRYLARVEAGEELGVTNKGRLVARLIPVQAAERSREALIESGVLIPARRPQNLLDVTAEPARGRKRT
LSDVLNEMRDEQ

Nucleotide

• Length: 279 bp

>AT85005 NZ_CP023627:3825970-3826248 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCCGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Structures

Toxin

• AlphaFold DB: G0TIR9