Detailed information of TA system    

experimentalExperimentally validated    

Overview

TA module


Type II Classification (family/domain) vapBC/RHH-PIN
Location 755335..756137 Replicon chromosome
Accession NC_000962
Organism Mycobacterium tuberculosis H37Rv

Toxin (Protein)


Gene name vapC Uniprot ID P9WFB3
Locus tag Rv0661c Protein ID NP_215175.1
Coordinates 755335..755772 (-) Length 146 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID O06777
Locus tag Rv0662c Protein ID NP_215176.2
Coordinates 755769..756023 (-) Length 85 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
Rv0655 751517..752596 + 1080 NP_215169.1 ABC transporter ATP-binding protein -
Rv0656c 752984..753367 - 384 NP_215170.1 ribonuclease VapC6 -
Rv0657c 753462..753617 - 156 NP_215171.1 antitoxin VapB6 -
Rv0658c 753693..754409 - 717 NP_215172.1 integral membrane protein -
Rv0659c 754685..754993 - 309 NP_215173.1 toxin MazF2 -
Rv0660c 754980..755225 - 246 NP_215174.1 antitoxin MazE2 -
Rv0661c 755335..755772 - 438 NP_215175.1 ribonuclease VapC7 Toxin
Rv0662c 755769..756023 - 255 NP_215176.2 antitoxin VapB7 Antitoxin
Rv0663 756137..758500 + 2364 NP_215177.1 arylsulfatase AtsD -
Rv0664 758532..758804 + 273 NP_215178.1 antitoxin VapB8 -
Rv0665 758801..759139 + 339 NP_215179.1 ribonuclease VapC8 -
Rv0666 759136..759309 + 174 NP_215180.1 membrane protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-126)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 146 a.a.        Molecular weight: 15245.39 Da        Isoelectric Point: 5.1865

>T85 NP_215175.1 NC_000962:c755772-755335 [Mycobacterium tuberculosis H37Rv]
MIVLDTTVLVYAKGAEHPLRDPCRDLVAAIADERIAATTTAEVIQEFVHVRARRRDRSDAAALGRVTMPNCSRRYSPSIE
ATSKRGLTLFETTPGLEACDAVLAAVAASAGATALVSADPAFADLSDVVHVIPDAAGMVSLLGDR

Download         Length: 438 bp

>T85 NC_000962:c755772-755335 [Mycobacterium tuberculosis H37Rv]
ATGATAGTGCTCGACACCACCGTCCTCGTCTATGCCAAAGGCGCCGAGCATCCACTCCGCGATCCCTGCCGCGATCTCGT
CGCGGCAATCGCCGACGAACGCATCGCTGCGACCACCACCGCCGAGGTGATCCAGGAGTTCGTCCACGTACGCGCCCGCC
GGCGCGACCGCAGCGACGCCGCCGCGCTGGGCCGTGTGACTATGCCGAACTGCTCTCGCCGCTACTCACCATCAATCGAG
GCCACCTCGAAGCGCGGCCTGACGCTGTTCGAGACAACGCCAGGGCTTGAAGCCTGCGACGCGGTGCTCGCGGCGGTTGC
CGCAAGCGCCGGCGCGACCGCTCTTGTCTCGGCTGACCCGGCGTTCGCCGATCTGTCGGATGTCGTGCACGTCATTCCCG
ACGCGGCCGGGATGGTCAGCCTGCTCGGCGACCGCTGA

Antitoxin


Download         Length: 85 a.a.        Molecular weight: 9450.99 Da        Isoelectric Point: 11.7073

>AT85 NP_215176.2 NC_000962:c756023-755769 [Mycobacterium tuberculosis H37Rv]
MSMRLAHRLQILLDDECHRRITAVARERGVPVATVVREAIDRGLVSPAGRRKSAGRRLLDAADMSVPEPRELKQELEALR
ARRG

Download         Length: 255 bp

>AT85 NC_000962:c756023-755769 [Mycobacterium tuberculosis H37Rv]
ATGAGCATGCGTCTGGCGCATCGGCTCCAGATCCTGCTCGACGACGAGTGCCACCGCCGCATCACCGCGGTGGCGCGCGA
GCGAGGCGTCCCGGTCGCAACCGTCGTGCGTGAGGCCATTGATCGAGGCCTCGTCAGCCCCGCCGGTCGCCGCAAGTCCG
CGGGCAGGCGCCTGCTCGACGCCGCCGACATGTCCGTTCCCGAGCCGCGGGAGCTGAAGCAGGAACTCGAGGCCCTCCGG
GCGCGCCGCGGATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB P9WFB3

Antitoxin

Source ID Structure
AlphaFold DB O06777

References

(1) Holly R Ramage et al. (2009) Comprehensive functional analysis of Mycobacterium tuberculosis toxin-antitoxin systems: implications for pathogenesis, stress responses, and evolution. PLoS Genetics 5(12):e1000767. [PubMed:20011113] experimental


experimental experimental literature