Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RelE-Phd
Location 3177273..3177821 Replicon chromosome
Accession NZ_CP023627
Organism Mycobacterium tuberculosis strain MDRMA2019

Toxin (Protein)


Gene name relE Uniprot ID G0TFG5
Locus tag CP483_RS15660 Protein ID WP_003414602.1
Coordinates 3177558..3177821 (+) Length 88 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID O33347
Locus tag CP483_RS15655 Protein ID WP_003414599.1
Coordinates 3177273..3177554 (+) Length 94 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP483_RS15630 3172896..3173753 - 858 WP_003899513.1 type I methionyl aminopeptidase -
CP483_RS15635 3173795..3174379 - 585 WP_003899514.1 DUF1707 domain-containing protein -
CP483_RS15640 3174483..3174731 + 249 WP_003913411.1 antitoxin VapB23 -
CP483_RS15645 3174728..3175108 + 381 WP_003414592.1 type II toxin-antitoxin system VapC family toxin -
CP483_RS15650 3175190..3177001 - 1812 WP_003906921.1 penicillin-binding protein -
CP483_RS15655 3177273..3177554 + 282 WP_003414599.1 type II toxin-antitoxin system antitoxin RelF Antitoxin
CP483_RS15660 3177558..3177821 + 264 WP_003414602.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
CP483_RS15665 3178194..3179048 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
CP483_RS15670 3179104..3180267 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
CP483_RS15675 3180284..3181498 - 1215 WP_003899518.1 zinc metalloprotease Rip -
CP483_RS15680 3181506..3182747 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(5-85)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-72)


Sequences

Toxin        


Download         Length: 88 a.a.        Molecular weight: 10204.82 Da        Isoelectric Point: 11.5078

>T84999 WP_003414602.1 NZ_CP023627:3177558-3177821 [Mycobacterium tuberculosis]
VPYTVRFTTTARRDLHKLPPRILAAVVEFAFGDLSREPLRVGKPLRRELAGTFSARRGTYRLLYRIDDEHTTVVILRVDH
RADIYRR

Download         Length: 264 bp

>T84999 NZ_CP023627:3177558-3177821 [Mycobacterium tuberculosis]
GTGCCTTACACCGTGCGGTTCACCACAACCGCGCGTCGAGACCTCCACAAGCTGCCACCGCGAATCCTCGCGGCAGTGGT
CGAATTCGCGTTCGGCGATCTGTCGCGCGAGCCCCTGCGGGTGGGCAAGCCCCTTCGGCGCGAGTTGGCCGGCACGTTCA
GCGCGCGTCGCGGAACGTACCGCCTGCTGTACCGGATTGACGACGAGCACACAACGGTAGTGATCCTGCGCGTCGATCAC
CGCGCGGACATCTACCGCCGATAG

Antitoxin


Download         Length: 94 a.a.        Molecular weight: 10183.40 Da        Isoelectric Point: 4.3440

>AT84999 WP_003414599.1 NZ_CP023627:3177273-3177554 [Mycobacterium tuberculosis]
MRILPISTIKGKLNEFVDAVSSTQDQITITKNGAPAAVLVGADEWESLQETLYWLAQPGIRESIAEADADIASGRTYGED
EIRAEFGVPRRPH

Download         Length: 282 bp

>AT84999 NZ_CP023627:3177273-3177554 [Mycobacterium tuberculosis]
ATGCGGATACTGCCGATTTCGACGATCAAGGGCAAGCTCAATGAGTTCGTCGACGCGGTCTCGTCGACACAGGACCAGAT
CACCATCACCAAGAACGGTGCACCCGCAGCCGTTCTGGTCGGCGCCGACGAGTGGGAATCGTTGCAGGAGACGCTGTACT
GGCTGGCGCAACCCGGAATCAGGGAGTCGATCGCTGAAGCCGACGCCGACATTGCCTCCGGCCGCACCTACGGCGAAGAC
GAGATCCGCGCCGAATTCGGCGTCCCGCGACGCCCCCACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 3G5O

Antitoxin

Source ID Structure
PDB 3G5O
AlphaFold DB A0A7U4BWP8

References