Detailed information of TA system TA084994

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3069897..3070576
• Replicon: chromosome
• Accession: NZ_CP023627
• Organism: Mycobacterium tuberculosis strain MDRMA2019

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: CP483_RS15095
• Protein ID: WP_003414059.1
• Coordinates: 3069897..3070313 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: CP483_RS15100
• Protein ID: WP_003414061.1
• Coordinates: 3070310..3070576 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP483_RS15075	3065949..3066851	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CP483_RS15080	3066920..3067672	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
CP483_RS15085	3067916..3068191	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
CP483_RS15090	3068188..3069810	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
CP483_RS15095	3069897..3070313	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
CP483_RS15100	3070310..3070576	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP483_RS15105	3070602..3070997	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
CP483_RS15110	3070994..3071263	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
CP483_RS15115	3071273..3072367	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
CP483_RS15120	3072364..3072783	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
CP483_RS15125	3072857..3073336	-	480	WP_003414073.1	dihydrofolate reductase	-
CP483_RS15130	3073407..3074207	-	801	WP_009939104.1	thymidylate synthase	-
CP483_RS15135	3074363..3075100	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T84994 WP_003414059.1 NZ_CP023627:c3070313-3069897 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T84994 NZ_CP023627:c3070313-3069897 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT84994 WP_003414061.1 NZ_CP023627:c3070576-3070310 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT84994 NZ_CP023627:c3070576-3070310 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64