Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2869456..2870093 Replicon chromosome
Accession NZ_CP023627
Organism Mycobacterium tuberculosis strain MDRMA2019

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag CP483_RS13965 Protein ID WP_003413180.1
Coordinates 2869456..2869851 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag CP483_RS13970 Protein ID WP_003413183.1
Coordinates 2869848..2870093 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP483_RS13920 2864859..2866070 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
CP483_RS13930 2866197..2866856 + 660 WP_003900846.1 LppA family lipoprotein -
CP483_RS13935 2866853..2867515 + 663 WP_003900848.1 hypothetical protein -
CP483_RS13940 2867512..2867790 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
CP483_RS13945 2867883..2868296 + 414 WP_003413164.1 PIN domain nuclease -
CP483_RS13950 2868335..2868592 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
CP483_RS13955 2868589..2868966 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
CP483_RS13960 2868982..2869356 - 375 WP_003413177.1 hypothetical protein -
CP483_RS13965 2869456..2869851 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
CP483_RS13970 2869848..2870093 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
CP483_RS13980 2870504..2870923 - 420 WP_003413190.1 A24 family peptidase -
CP483_RS13985 2870935..2871744 - 810 WP_003413193.1 shikimate dehydrogenase -
CP483_RS13990 2871741..2872994 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
CP483_RS13995 2872987..2873499 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T84991 WP_003413180.1 NZ_CP023627:c2869851-2869456 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T84991 NZ_CP023627:c2869851-2869456 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT84991 WP_003413183.1 NZ_CP023627:c2870093-2869848 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT84991 NZ_CP023627:c2870093-2869848 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References