Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2807812..2808464 Replicon chromosome
Accession NZ_CP023627
Organism Mycobacterium tuberculosis strain MDRMA2019

Toxin (Protein)


Gene name vapC Uniprot ID G0TPX1
Locus tag CP483_RS13630 Protein ID WP_003412752.1
Coordinates 2808039..2808464 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag CP483_RS13625 Protein ID WP_003412749.1
Coordinates 2807812..2808033 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP483_RS13610 2806052..2806258 - 207 WP_003899347.1 hypothetical protein -
CP483_RS13615 2806394..2807017 + 624 WP_003900858.1 TIGR00725 family protein -
CP483_RS13620 2807007..2807759 + 753 WP_003900859.1 hypothetical protein -
CP483_RS13625 2807812..2808033 + 222 WP_003412749.1 antitoxin Antitoxin
CP483_RS13630 2808039..2808464 + 426 WP_003412752.1 type II toxin-antitoxin system VapC family toxin Toxin
CP483_RS13635 2808487..2809668 - 1182 WP_003899351.1 2-oxo acid dehydrogenase subunit E2 -
CP483_RS13640 2809665..2810711 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
CP483_RS13645 2810722..2811825 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
CP483_RS13650 2812084..2812905 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
CP483_RS13655 2812902..2813459 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15392.89 Da        Isoelectric Point: 9.2386

>T84987 WP_003412752.1 NZ_CP023627:2808039-2808464 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T84987 NZ_CP023627:2808039-2808464 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT84987 WP_003412749.1 NZ_CP023627:2807812-2808033 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT84987 NZ_CP023627:2807812-2808033 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TPX1

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References