Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1686073..1686689 Replicon chromosome
Accession NZ_CP023627
Organism Mycobacterium tuberculosis strain MDRMA2019

Toxin (Protein)


Gene name mazF Uniprot ID G0TJ74
Locus tag CP483_RS08185 Protein ID WP_003407593.1
Coordinates 1686372..1686689 (+) Length 106 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TJ73
Locus tag CP483_RS08180 Protein ID WP_003900349.1
Coordinates 1686073..1686375 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP483_RS08170 1681959..1683806 + 1848 WP_003407585.1 methylmalonyl-CoA mutase small subunit -
CP483_RS08175 1683807..1686059 + 2253 WP_003407587.1 methylmalonyl-CoA mutase -
CP483_RS08180 1686073..1686375 + 303 WP_003900349.1 type II toxin-antitoxin system antitoxin MazE4 Antitoxin
CP483_RS08185 1686372..1686689 + 318 WP_003407593.1 type II toxin-antitoxin system toxin endoribonuclease MazF4 Toxin
CP483_RS08190 1686686..1687690 + 1005 WP_003407596.1 methylmalonyl Co-A mutase-associated GTPase MeaB -
CP483_RS08195 1687743..1689032 + 1290 WP_003407599.1 serine hydrolase -
CP483_RS08200 1689105..1689878 - 774 WP_003912632.1 class I SAM-dependent methyltransferase -
CP483_RS08205 1689936..1690148 - 213 WP_003898900.1 dodecin family protein -
CP483_RS21790 1690158..1690382 - 225 WP_160522526.1 NUDIX domain-containing protein -
CP483_RS08215 1690652..1691680 + 1029 WP_003906616.1 glycosyltransferase family 2 protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(6-99)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 11377.97 Da        Isoelectric Point: 7.9950

>T84973 WP_003407593.1 NZ_CP023627:1686372-1686689 [Mycobacterium tuberculosis]
VNAPLRGQVYRCDLGYGAKPWLIVSNNARNRHTADVVAVRLTTTRRTIPTWVAMGPSDPLTGYVNADNIETLGKDELGDY
LGEVTPATMNKINTALATALGLPWP

Download         Length: 318 bp

>T84973 NZ_CP023627:1686372-1686689 [Mycobacterium tuberculosis]
GTGAACGCGCCGTTGCGTGGTCAGGTCTATCGATGCGACCTCGGATACGGGGCCAAACCGTGGCTCATCGTCTCCAACAA
CGCCCGCAACCGTCACACCGCCGACGTGGTGGCTGTGCGCCTGACAACAACGCGGAGAACCATACCGACCTGGGTCGCCA
TGGGCCCCAGCGATCCATTGACCGGATACGTCAACGCGGACAACATCGAGACCCTCGGCAAAGACGAGCTCGGTGACTAC
CTCGGTGAGGTCACGCCGGCGACGATGAACAAAATCAACACGGCGCTCGCGACCGCGCTGGGGCTACCGTGGCCATGA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11173.42 Da        Isoelectric Point: 9.5564

>AT84973 WP_003900349.1 NZ_CP023627:1686073-1686375 [Mycobacterium tuberculosis]
VPFLVALSGIISGVRDHSMTVRLDQQTRQRLQDIVKGGYRSANAAIVDAINKRWEALHDEQLDAAYAAAIHDNPAYPYES
EAERSAARARRNARQQRSAQ

Download         Length: 303 bp

>AT84973 NZ_CP023627:1686073-1686375 [Mycobacterium tuberculosis]
GTGCCGTTTCTGGTTGCATTATCCGGTATCATCTCGGGCGTGCGTGATCATTCGATGACCGTGCGGCTCGACCAGCAAAC
TCGCCAGCGCCTGCAAGACATTGTGAAAGGCGGATACCGGAGCGCTAATGCGGCGATCGTCGACGCCATCAACAAGCGCT
GGGAGGCGCTACACGATGAGCAACTCGACGCCGCCTACGCGGCCGCGATCCATGACAATCCGGCGTACCCGTACGAGTCT
GAGGCCGAACGGAGCGCCGCGCGGGCCCGGCGCAACGCCAGGCAGCAGCGCTCGGCACAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5XE3
PDB 5XE2

Antitoxin

Source ID Structure
PDB 5XE3
AlphaFold DB G0TJ73

References