Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1230465..1231096 Replicon chromosome
Accession NZ_CP023627
Organism Mycobacterium tuberculosis strain MDRMA2019

Toxin (Protein)


Gene name mazF Uniprot ID P9WIH8
Locus tag CP483_RS06040 Protein ID WP_003898728.1
Coordinates 1230465..1230776 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TGZ7
Locus tag CP483_RS06045 Protein ID WP_003405836.1
Coordinates 1230776..1231096 (-) Length 107 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP483_RS06020 1225946..1227370 - 1425 WP_003405805.1 class II fumarate hydratase -
CP483_RS06025 1227401..1228489 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
CP483_RS06030 1228545..1229189 + 645 WP_003915494.1 DUF4245 domain-containing protein -
CP483_RS06035 1229196..1230353 - 1158 WP_003898727.1 AI-2E family transporter -
CP483_RS06040 1230465..1230776 - 312 WP_003898728.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
CP483_RS06045 1230776..1231096 - 321 WP_003405836.1 type II toxin-antitoxin system antitoxin MazE3 Antitoxin
CP483_RS06050 1231106..1232631 + 1526 Protein_1148 carboxylesterase/lipase family protein -
CP483_RS06055 1232649..1233761 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
CP483_RS06060 1233771..1234028 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
CP483_RS06065 1234018..1235265 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
CP483_RS06070 1235262..1235900 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11049.76 Da        Isoelectric Point: 4.9371

>T84967 WP_003898728.1 NZ_CP023627:c1230776-1230465 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNTQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T84967 NZ_CP023627:c1230776-1230465 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACACCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 107 a.a.        Molecular weight: 11394.83 Da        Isoelectric Point: 5.1236

>AT84967 WP_003405836.1 NZ_CP023627:c1231096-1230776 [Mycobacterium tuberculosis]
MYLPWGVVLAGGANGFGAGAYQTGTICEVSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEI
LATNTSATGDLDTLAGHCARTALDID

Download         Length: 321 bp

>AT84967 NZ_CP023627:c1231096-1230776 [Mycobacterium tuberculosis]
ATGTACCTACCCTGGGGGGTCGTCCTCGCCGGCGGCGCGAACGGTTTTGGGGCGGGTGCATACCAAACTGGTACCATCTG
TGAAGTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATG
CCCGTAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATC
CTCGCCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTG
A

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5CCA
PDB 5UCT

Antitoxin

Source ID Structure
AlphaFold DB G0TGZ7

References