Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 754505..755045 Replicon chromosome
Accession NZ_CP023627
Organism Mycobacterium tuberculosis strain MDRMA2019

Toxin (Protein)


Gene name mazF Uniprot ID P9WII0
Locus tag CP483_RS03545 Protein ID WP_003403376.1
Coordinates 754505..754813 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID G0TQE0
Locus tag CP483_RS03550 Protein ID WP_003403381.1
Coordinates 754800..755045 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP483_RS03520 749820..751325 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
CP483_RS03525 751406..752416 + 1011 WP_009937374.1 ABC transporter ATP-binding protein -
CP483_RS03530 752804..753187 - 384 WP_003403365.1 ribonuclease VapC6 -
CP483_RS03535 753282..753437 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin -
CP483_RS03540 753513..754229 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
CP483_RS03545 754505..754813 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin Toxin
CP483_RS03550 754800..755045 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family Antitoxin
CP483_RS03555 755155..755592 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
CP483_RS03560 755589..755843 - 255 WP_003911263.1 antitoxin VapB7 -
CP483_RS03565 755957..758320 + 2364 WP_003898547.1 arylsulfatase AtsD -
CP483_RS03570 758383..758709 + 327 WP_003403401.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CP483_RS03575 758621..758959 + 339 WP_003403405.1 PIN domain-containing protein -
CP483_RS03580 758956..759129 + 174 WP_003898549.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(1-101)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11320.10 Da        Isoelectric Point: 11.6554

>T84960 WP_003403376.1 NZ_CP023627:c754813-754505 [Mycobacterium tuberculosis]
MRRGELWFAATPGGDRPVLVLTRDPVADRIGAVVVVALTRTRRGLVSELELTAVENRVPSDCVVNFDNIHTLPRTAFRRR
ITRLSPARLHEACQTLRASTGC

Download         Length: 309 bp

>T84960 NZ_CP023627:c754813-754505 [Mycobacterium tuberculosis]
ATGCGGCGCGGTGAATTGTGGTTTGCCGCCACACCTGGTGGTGACAGACCAGTACTTGTCCTTACCAGAGATCCGGTGGC
AGACCGCATCGGCGCGGTCGTTGTGGTGGCCCTAACCCGCACCCGCCGAGGCCTGGTGTCGGAATTGGAGCTCACGGCCG
TCGAAAACCGTGTTCCGAGCGACTGCGTCGTCAACTTCGACAACATTCATACGTTGCCACGCACCGCATTCCGACGCCGC
ATCACCCGGCTGTCCCCGGCCCGCCTGCACGAAGCCTGTCAAACACTCCGGGCGAGCACGGGGTGTTGA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9169.01 Da        Isoelectric Point: 4.2070

>AT84960 WP_003403381.1 NZ_CP023627:c755045-754800 [Mycobacterium tuberculosis]
MLSFRADDHDVDLADAWARRLHIGRSELLRDALRRHLAALAADQDVQAYTERPLTDDENALAEIADWGPAEDWADWADAA
R

Download         Length: 246 bp

>AT84960 NZ_CP023627:c755045-754800 [Mycobacterium tuberculosis]
ATGCTCAGCTTCCGCGCCGACGACCACGACGTCGACCTGGCCGACGCGTGGGCGCGTCGCCTACACATCGGCCGCTCCGA
GCTGCTGCGCGATGCGCTGCGACGTCACTTGGCCGCGCTGGCCGCGGACCAGGATGTCCAGGCCTACACCGAGCGACCGT
TGACGGACGACGAGAACGCACTGGCTGAGATCGCCGACTGGGGGCCGGCGGAGGACTGGGCCGACTGGGCCGATGCGGCG
CGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSE5

Antitoxin

Source ID Structure
AlphaFold DB G0TQE0

References