Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2807812..2808464 Replicon chromosome
Accession NZ_CP023626
Organism Mycobacterium tuberculosis strain MDRMA1565

Toxin (Protein)


Gene name vapC Uniprot ID A0A5R1ZCY8
Locus tag CP482_RS13625 Protein ID WP_003905869.1
Coordinates 2808039..2808464 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag CP482_RS13620 Protein ID WP_003412749.1
Coordinates 2807812..2808033 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP482_RS13605 2806052..2806258 - 207 WP_003899347.1 hypothetical protein -
CP482_RS13610 2806394..2807017 + 624 WP_003900858.1 TIGR00725 family protein -
CP482_RS13615 2807007..2807759 + 753 WP_003901416.1 hypothetical protein -
CP482_RS13620 2807812..2808033 + 222 WP_003412749.1 antitoxin Antitoxin
CP482_RS13625 2808039..2808464 + 426 WP_003905869.1 type II toxin-antitoxin system VapC family toxin Toxin
CP482_RS13630 2808487..2809668 - 1182 WP_003905870.1 2-oxo acid dehydrogenase subunit E2 -
CP482_RS13635 2809665..2810711 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
CP482_RS13640 2810722..2811825 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
CP482_RS13645 2812084..2812905 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
CP482_RS13650 2812902..2813459 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-121)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15364.84 Da        Isoelectric Point: 9.2386

>T84927 WP_003905869.1 NZ_CP023626:2808039-2808464 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRASTNPKVLPSAIGIADARRVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T84927 NZ_CP023626:2808039-2808464 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGCGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT84927 WP_003412749.1 NZ_CP023626:2807812-2808033 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT84927 NZ_CP023626:2807812-2808033 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5R1ZCY8

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References