Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 1764522..1765135 Replicon chromosome
Accession NZ_CP023626
Organism Mycobacterium tuberculosis strain MDRMA1565

Toxin (Protein)


Gene name vapC Uniprot ID P64880
Locus tag CP482_RS08535 Protein ID WP_003407786.1
Coordinates 1764731..1765135 (+) Length 135 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A0E8UJ46
Locus tag CP482_RS08530 Protein ID WP_009935474.1
Coordinates 1764522..1764725 (+) Length 68 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP482_RS08500 1759927..1760307 + 381 WP_003407768.1 fumarate reductase subunit C -
CP482_RS08505 1760304..1760681 + 378 WP_003407771.1 fumarate reductase subunit FrdD -
CP482_RS08510 1760749..1761357 + 609 WP_003407773.1 TetR/AcrR family transcriptional regulator -
CP482_RS08515 1761541..1762689 + 1149 Protein_1611 MMPL family transporter -
CP482_RS08520 1762699..1763145 + 447 WP_003407780.1 F420H(2)-dependent quinone reductase -
CP482_RS08525 1763180..1764469 + 1290 WP_003407781.1 threonine ammonia-lyase -
CP482_RS08530 1764522..1764725 + 204 WP_009935474.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP482_RS08535 1764731..1765135 + 405 WP_003407786.1 type II toxin-antitoxin system toxin ribonuclease VapC11 Toxin
CP482_RS08540 1765152..1766894 - 1743 WP_003407788.1 malto-oligosyltrehalose trehalohydrolase -
CP482_RS08545 1766887..1769184 - 2298 WP_003407790.1 malto-oligosyltrehalose synthase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(2-124)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-44)


Sequences

Toxin        


Download         Length: 135 a.a.        Molecular weight: 14650.76 Da        Isoelectric Point: 5.6587

>T84913 WP_003407786.1 NZ_CP023626:1764731-1765135 [Mycobacterium tuberculosis]
MILIDTSAWVEYFRATGSIAAVEVRRLLSEEAARIAMCEPIAMEILSGALDDNTHTTLERLVNGLPSLNVDDAIDFRAAA
GIYRAARRAGETVRSINDCLIAALAIRHGARIVHRDADFDVIARITNLQAASFR

Download         Length: 405 bp

>T84913 NZ_CP023626:1764731-1765135 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin


Download         Length: 68 a.a.        Molecular weight: 7501.48 Da        Isoelectric Point: 4.3951

>AT84913 WP_009935474.1 NZ_CP023626:1764522-1764725 [Mycobacterium tuberculosis]
MSRTNIDIDDELAAEVMRRFGLTTKRAAVDLALRRLVGSPLSREFLLGLEGVGWEGDLDDLRSDRPD

Download         Length: 204 bp

>AT84913 NZ_CP023626:1764522-1764725 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6A7V

Antitoxin

Source ID Structure
AlphaFold DB A0A0E8UJ46

References