Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 752903..753536 Replicon chromosome
Accession NZ_CP023626
Organism Mycobacterium tuberculosis strain MDRMA1565

Toxin (Protein)


Gene name vapC Uniprot ID G0TQD6
Locus tag CP482_RS03525 Protein ID WP_003403365.1
Coordinates 752903..753286 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ56
Locus tag CP482_RS03530 Protein ID WP_003403368.1
Coordinates 753381..753536 (-) Length 52 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP482_RS03500 748195..748731 + 537 WP_003403341.1 50S ribosomal protein L10 -
CP482_RS03505 748768..749160 + 393 WP_003403353.1 50S ribosomal protein L7/L12 -
CP482_RS03510 749153..749848 - 696 WP_003403355.1 TetR/AcrR family transcriptional regulator -
CP482_RS03515 749919..751424 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
CP482_RS03520 751505..752515 + 1011 WP_003911248.1 ABC transporter ATP-binding protein -
CP482_RS03525 752903..753286 - 384 WP_003403365.1 ribonuclease VapC6 Toxin
CP482_RS03530 753381..753536 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP482_RS03535 753612..754328 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
CP482_RS03540 754604..754912 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
CP482_RS03545 754899..755144 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family -
CP482_RS03550 755254..755691 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
CP482_RS03555 755688..755942 - 255 WP_003911263.1 antitoxin VapB7 -
CP482_RS03560 756056..758419 + 2364 WP_003901895.1 arylsulfatase AtsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13959.73 Da        Isoelectric Point: 6.0803

>T84899 WP_003403365.1 NZ_CP023626:c753286-752903 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Download         Length: 384 bp

>T84899 NZ_CP023626:c753286-752903 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 52 a.a.        Molecular weight: 5872.72 Da        Isoelectric Point: 5.7734

>AT84899 WP_003403368.1 NZ_CP023626:c753536-753381 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Download         Length: 156 bp

>AT84899 NZ_CP023626:c753536-753381 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQD6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSF8

References