Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3707506..3708180 Replicon chromosome
Accession NZ_CP023623
Organism Mycobacterium tuberculosis strain MDRMA203

Toxin (Protein)


Gene name vapC Uniprot ID P9WF52
Locus tag CP479_RS18170 Protein ID WP_003417282.1
Coordinates 3707506..3707934 (-) Length 143 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TI59
Locus tag CP479_RS18175 Protein ID WP_003417286.1
Coordinates 3707938..3708180 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP479_RS18145 3703328..3703729 - 402 WP_003417264.1 cytidine deaminase -
CP479_RS18150 3703870..3704304 + 435 WP_003900017.1 succinate dehydrogenase, cytochrome b556 subunit -
CP479_RS18155 3704301..3704735 + 435 WP_003417269.1 succinate dehydrogenase hydrophobic membrane anchor subunit -
CP479_RS18160 3704864..3706636 + 1773 WP_003417273.1 succinate dehydrogenase flavoprotein subunit -
CP479_RS18165 3706636..3707427 + 792 WP_003417279.1 succinate dehydrogenase iron-sulfur subunit -
CP479_RS18170 3707506..3707934 - 429 WP_003417282.1 type II toxin-antitoxin system VapC family toxin Toxin
CP479_RS18175 3707938..3708180 - 243 WP_003417286.1 ribbon-helix-helix domain-containing protein Antitoxin
CP479_RS18180 3708302..3708916 - 615 WP_003417288.1 class I SAM-dependent methyltransferase -
CP479_RS18185 3708913..3709578 - 666 WP_003417290.1 molybdopterin converting factor subunit 1 -
CP479_RS18190 3709579..3710112 - 534 WP_003417293.1 cyclic pyranopterin monophosphate synthase MoaC -
CP479_RS18195 3710109..3710198 - 90 WP_009934953.1 pterin-4-alpha-carbinolamine dehydratase moaB3 -
CP479_RS18200 3710298..3711559 + 1262 WP_087902221.1 IS3-like element IS987 family transposase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-141)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 143 a.a.        Molecular weight: 15834.16 Da        Isoelectric Point: 8.5471

>T84761 WP_003417282.1 NZ_CP023623:c3707934-3707506 [Mycobacterium tuberculosis]
MRALLDVNVLLALLDRDHVDHERARAWITGQIERGWASCAITQNGFVRVISQPRYPSPISVAHAIDLLARATHTRYHEFW
SCTVSILDSKVIDRSRLHSPKQVTDAYLLALAVAHDGRFVTFDQSIALTAVPGATKQHLATL

Download         Length: 429 bp

>T84761 NZ_CP023623:c3707934-3707506 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 8849.86 Da        Isoelectric Point: 5.1007

>AT84761 WP_003417286.1 NZ_CP023623:c3708180-3707938 [Mycobacterium tuberculosis]
MRTTLSIDDDVLLAVKERARREKRTAGEILSDLARQALTNQNPQPAASQEDAFHGFEPLPHRGGAVSNALIDRLRDEEAV

Download         Length: 243 bp

>AT84761 NZ_CP023623:c3708180-3707938 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4FBL0

Antitoxin

Source ID Structure
AlphaFold DB G0TI59

References