Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 3110059..3110629 Replicon chromosome
Accession NZ_CP023623
Organism Mycobacterium tuberculosis strain MDRMA203

Toxin (Protein)


Gene name mazF Uniprot ID P71650
Locus tag CP479_RS15310 Protein ID WP_003414166.1
Coordinates 3110059..3110415 (-) Length 119 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID P0CL61
Locus tag CP479_RS15315 Protein ID WP_003901465.1
Coordinates 3110399..3110629 (-) Length 77 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP479_RS15290 3105511..3107199 - 1689 WP_003414155.1 alpha/beta hydrolase family protein -
CP479_RS15295 3107203..3107529 - 327 WP_003414157.1 hypothetical protein -
CP479_RS15300 3107702..3108289 + 588 WP_003914429.1 DUF3558 family protein -
CP479_RS15305 3108308..3109957 + 1650 WP_003900576.1 CocE/NonD family hydrolase -
CP479_RS15310 3110059..3110415 - 357 WP_003414166.1 type II toxin-antitoxin system toxin endoribonuclease MazF9 Toxin
CP479_RS15315 3110399..3110629 - 231 WP_003901465.1 antitoxin MazE Antitoxin
CP479_RS15320 3110672..3111715 - 1044 WP_003414172.1 DUF2293 domain-containing protein -
CP479_RS15325 3111906..3112181 + 276 WP_003911993.1 DUF1778 domain-containing protein -
CP479_RS21530 3112357..3112611 - 255 WP_003917684.1 hypothetical protein -
CP479_RS15335 3112759..3113163 + 405 WP_009938577.1 hypothetical protein -
CP479_RS15340 3113160..3113351 + 192 WP_003414184.1 hypothetical protein -
CP479_RS15345 3113415..3114704 + 1290 Protein_2900 transposase family protein -
CP479_RS15350 3114938..3115195 + 258 WP_003899489.1 hypothetical protein -
CP479_RS15355 3115300..3115611 + 312 WP_003414190.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(3-113)

Antitoxin

Ribbon-helix-helix protein, CopG

(3-29)


Sequences

Toxin        


Download         Length: 119 a.a.        Molecular weight: 12858.73 Da        Isoelectric Point: 9.1962

>T84755 WP_003414166.1 NZ_CP023623:c3110415-3110059 [Mycobacterium tuberculosis]
VMRRGEIWQVDLDPARGSEANNQRPAVVVSNDRANATATRLGRGVITVVPVTSNIAKVYPFQVLLSATTTGLQVDCKAQA
EQIRSIATERLLRPIGRVSAAELAQLDEALKLHLDLWS

Download         Length: 357 bp

>T84755 NZ_CP023623:c3110415-3110059 [Mycobacterium tuberculosis]
GTGATGCGCCGCGGTGAGATTTGGCAGGTCGATCTCGACCCCGCTCGAGGTAGCGAAGCGAACAACCAGCGCCCCGCCGT
CGTCGTCAGCAACGACCGGGCCAACGCGACCGCCACGCGTCTTGGGCGCGGCGTCATCACCGTCGTGCCGGTGACGAGCA
ACATCGCCAAGGTCTATCCGTTTCAGGTGTTGTTGTCGGCCACCACTACTGGTCTCCAGGTCGACTGCAAGGCGCAGGCC
GAGCAAATCAGATCGATTGCTACCGAGCGGTTGCTCCGGCCAATCGGCCGAGTTTCAGCCGCCGAACTTGCCCAGCTCGA
TGAGGCTTTGAAACTGCATCTCGACTTATGGTCGTAG

Antitoxin


Download         Length: 77 a.a.        Molecular weight: 8231.01 Da        Isoelectric Point: 4.1809

>AT84755 WP_003901465.1 NZ_CP023623:c3110629-3110399 [Mycobacterium tuberculosis]
VKLSVSLSDDDVAILDAYVKRAGLPSRSAGLQHAIRVLRYPTLEDDYANAWQEWSAAGDTDAWEQTVGDGVGDAPR

Download         Length: 231 bp

>AT84755 NZ_CP023623:c3110629-3110399 [Mycobacterium tuberculosis]
GTGAAGTTGAGCGTGAGCCTGTCCGATGACGACGTCGCGATCCTGGACGCATATGTGAAACGAGCGGGATTACCATCCCG
CTCCGCCGGCCTGCAGCATGCGATCCGCGTGCTTCGCTACCCCACGCTCGAAGATGACTATGCCAACGCATGGCAAGAAT
GGTCGGCAGCCGGCGACACGGACGCGTGGGAGCAGACCGTCGGCGACGGAGTCGGTGATGCGCCGCGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6L2A
PDB 5HJZ
PDB 6KYT
PDB 6L29
PDB 7DU5
PDB 6KYS

Antitoxin

Source ID Structure
PDB 7DU5
PDB 6KYT
AlphaFold DB A0A7U4FBW3

References