Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2868500..2869131 Replicon chromosome
Accession NZ_CP023623
Organism Mycobacterium tuberculosis strain MDRMA203

Toxin (Protein)


Gene name vapC Uniprot ID G0TQ28
Locus tag CP479_RS13945 Protein ID WP_003413174.1
Coordinates 2868754..2869131 (+) Length 126 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P95006
Locus tag CP479_RS13940 Protein ID WP_003413167.1
Coordinates 2868500..2868757 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP479_RS13905 2864414..2864728 + 315 WP_009937839.1 hypothetical protein -
CP479_RS13910 2865024..2866235 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
CP479_RS13920 2866362..2867021 + 660 WP_096874637.1 LppA family lipoprotein -
CP479_RS13925 2867018..2867680 + 663 WP_077553666.1 hypothetical protein -
CP479_RS13930 2867677..2867955 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
CP479_RS13935 2868048..2868461 + 414 WP_003413164.1 PIN domain nuclease -
CP479_RS13940 2868500..2868757 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein Antitoxin
CP479_RS13945 2868754..2869131 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin Toxin
CP479_RS13950 2869147..2869521 - 375 WP_003413177.1 hypothetical protein -
CP479_RS13955 2869621..2870016 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 -
CP479_RS13960 2870013..2870258 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 -
CP479_RS13970 2870669..2871088 - 420 WP_003413190.1 A24 family peptidase -
CP479_RS13975 2871100..2871909 - 810 WP_003413193.1 shikimate dehydrogenase -
CP479_RS13980 2871906..2873159 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
CP479_RS13985 2873152..2873664 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(3-111)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 126 a.a.        Molecular weight: 13728.72 Da        Isoelectric Point: 4.4687

>T84749 WP_003413174.1 NZ_CP023623:2868754-2869131 [Mycobacterium tuberculosis]
VKLIDTTIAVDHLRGEPAAAVLLAELINNGEEIAASELVRFELLAGVRESELAALEAFFSAVVWTLVTEDIARIGGRLAR
RYRSSHRGIDDVDYLIAATAIVVDADLLTTNVRHFPMFPDLQPPY

Download         Length: 378 bp

>T84749 NZ_CP023623:2868754-2869131 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9453.63 Da        Isoelectric Point: 10.6555

>AT84749 WP_003413167.1 NZ_CP023623:2868500-2868757 [Mycobacterium tuberculosis]
MRTQVTLGKEELELLDRAAKASGASRSELIRRAIHRAYGTGSKQERLAALDHSRGSWRGRDFTGTEYVDAIRGDLNERLA
RLGLA

Download         Length: 258 bp

>AT84749 NZ_CP023623:2868500-2868757 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQ28

Antitoxin

Source ID Structure
AlphaFold DB A0A829C9W5

References