Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2807977..2808629 Replicon chromosome
Accession NZ_CP023623
Organism Mycobacterium tuberculosis strain MDRMA203

Toxin (Protein)


Gene name vapC Uniprot ID Q7D717
Locus tag CP479_RS13620 Protein ID WP_003900860.1
Coordinates 2808204..2808629 (+) Length 142 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ24
Locus tag CP479_RS13615 Protein ID WP_003412749.1
Coordinates 2807977..2808198 (+) Length 74 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP479_RS13600 2806217..2806423 - 207 WP_003899347.1 hypothetical protein -
CP479_RS13605 2806559..2807182 + 624 WP_003900858.1 TIGR00725 family protein -
CP479_RS13610 2807172..2807924 + 753 WP_003900859.1 hypothetical protein -
CP479_RS13615 2807977..2808198 + 222 WP_003412749.1 antitoxin Antitoxin
CP479_RS13620 2808204..2808629 + 426 WP_003900860.1 type II toxin-antitoxin system VapC family toxin Toxin
CP479_RS13625 2808652..2809833 - 1182 WP_003899351.1 2-oxo acid dehydrogenase subunit E2 -
CP479_RS13630 2809830..2810876 - 1047 WP_003412757.1 3-methyl-2-oxobutanoate dehydrogenase subunit beta -
CP479_RS13635 2810887..2811990 - 1104 WP_003412761.1 pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha -
CP479_RS13640 2812249..2813070 - 822 WP_003412766.1 citrate (pro-3S)-lyase subunit beta -
CP479_RS13645 2813067..2813624 - 558 WP_003412768.1 MaoC family dehydratase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(2-139)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 142 a.a.        Molecular weight: 15333.82 Da        Isoelectric Point: 8.1220

>T84746 WP_003900860.1 NZ_CP023623:2808204-2808629 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARPVLVALRAVGGHRFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Download         Length: 426 bp

>T84746 NZ_CP023623:2808204-2808629 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACCGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin


Download         Length: 74 a.a.        Molecular weight: 7864.93 Da        Isoelectric Point: 4.5258

>AT84746 WP_003412749.1 NZ_CP023623:2807977-2808198 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Download         Length: 222 bp

>AT84746 NZ_CP023623:2807977-2808198 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E7ZFR7

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW03

References