Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2225333..2226021 Replicon chromosome
Accession NZ_CP023623
Organism Mycobacterium tuberculosis strain MDRMA203

Toxin (Protein)


Gene name vapC Uniprot ID P9WF64
Locus tag CP479_RS10765 Protein ID WP_003899117.1
Coordinates 2225333..2225752 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag CP479_RS10770 Protein ID WP_003409968.1
Coordinates 2225761..2226021 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP479_RS10745 2220828..2221676 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
CP479_RS10750 2221639..2223084 - 1446 WP_003899115.1 APC family permease -
CP479_RS10755 2223263..2223949 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
CP479_RS10760 2224140..2225108 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
CP479_RS10765 2225333..2225752 - 420 WP_003899117.1 type II toxin-antitoxin system VapC family toxin Toxin
CP479_RS10770 2225761..2226021 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP479_RS10775 2226164..2227840 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
CP479_RS10780 2227828..2228481 - 654 WP_003409976.1 cutinase Cfp21 -
CP479_RS10785 2228596..2228826 + 231 WP_003409980.1 DUF167 domain-containing protein -
CP479_RS10790 2228911..2229822 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
CP479_RS10795 2229931..2230530 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14748.87 Da        Isoelectric Point: 7.9722

>T84738 WP_003899117.1 NZ_CP023623:c2225752-2225333 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDHEFRGVVGYWPGVA

Download         Length: 420 bp

>T84738 NZ_CP023623:c2225752-2225333 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCATGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT84738 WP_003409968.1 NZ_CP023623:c2226021-2225761 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT84738 NZ_CP023623:c2226021-2225761 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E7TLI0

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References