Detailed information of TA system TA084693

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3070372..3071059
• Replicon: chromosome
• Accession: NZ_CP023622
• Organism: Mycobacterium tuberculosis strain MDRDM827

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TF65
• Locus tag: CP478_RS15115
• Protein ID: WP_003414064.1
• Coordinates: 3070664..3071059 (-)
• Length: 132 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: CP478_RS15110
• Protein ID: WP_003414061.1
• Coordinates: 3070372..3070638 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP478_RS15085	3066011..3066913	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CP478_RS15090	3066982..3067734	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
CP478_RS15095	3067978..3068253	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
CP478_RS15100	3068250..3069872	-	1623	WP_003906897.1	type I restriction-modification system subunit M	-
CP478_RS15105	3069959..3070375	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	-
CP478_RS15110	3070372..3070638	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP478_RS15115	3070664..3071059	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP478_RS15120	3071056..3071325	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
CP478_RS15125	3071335..3072429	-	1095	WP_003414068.1	restriction endonuclease subunit S	-
CP478_RS15130	3072426..3072845	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
CP478_RS15135	3072919..3073398	-	480	WP_003414073.1	dihydrofolate reductase	-
CP478_RS15140	3073469..3074269	-	801	WP_009939104.1	thymidylate synthase	-
CP478_RS15145	3074425..3075162	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 132 a.a.
• Molecular weight: 14340.04 Da
• Isoelectric Point: 4.7145

>T84693 WP_003414064.1 NZ_CP023622:c3071059-3070664 [Mycobacterium tuberculosis]
VIVDTSAIVAIVSGESGAQVLKEALERSPNSRMSAPNYVELCAIMQRRDRPEISRLVDRLLDDYGIQVEAVDADQARVAA
QAYRDYGRGSGHPARLNLGDTYSYALAQVTGEPLLFRGDDFTHTDIRPACT

Nucleotide

• Length: 396 bp

>T84693 NZ_CP023622:c3071059-3070664 [Mycobacterium tuberculosis]
GTGATCGTCGATACGTCGGCCATCGTCGCCATCGTGAGCGGGGAATCGGGCGCGCAGGTGCTCAAGGAGGCGCTGGAGCG
GTCACCGAACTCCCGAATGTCCGCGCCCAACTACGTCGAACTGTGCGCGATCATGCAGCGGCGGGACCGGCCGGAGATCT
CTCGATTGGTGGACCGTTTGCTGGACGACTACGGAATCCAGGTCGAAGCCGTCGACGCCGACCAAGCCCGCGTGGCCGCG
CAGGCGTATCGCGACTACGGCCGCGGCAGCGGCCATCCGGCCCGTCTCAACCTCGGCGACACCTACAGCTACGCCCTGGC
CCAGGTCACCGGGGAGCCGCTGCTGTTTCGTGGCGATGACTTCACGCATACCGATATCCGGCCCGCGTGCACCTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT84693 WP_003414061.1 NZ_CP023622:c3070638-3070372 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT84693 NZ_CP023622:c3070638-3070372 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• AlphaFold DB: G0TF65

Antitoxin

• AlphaFold DB: G0TF64