Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2929859..2930573 Replicon chromosome
Accession NZ_CP023622
Organism Mycobacterium tuberculosis strain MDRDM827

Toxin (Protein)


Gene name vapC Uniprot ID G0TQK0
Locus tag CP478_RS14260 Protein ID WP_003413460.1
Coordinates 2930133..2930573 (+) Length 147 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ20
Locus tag CP478_RS14255 Protein ID WP_003413456.1
Coordinates 2929859..2930146 (+) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP478_RS14220 2925281..2925526 + 246 WP_003413429.1 AbrB/MazE/SpoVT family DNA-binding domain-containing protein -
CP478_RS14225 2925523..2925927 + 405 WP_003899389.1 type II toxin-antitoxin system VapC family toxin -
CP478_RS14230 2926144..2926764 + 621 WP_003413441.1 DUF4178 domain-containing protein -
CP478_RS14235 2926775..2927269 + 495 WP_003413444.1 DUF2617 family protein -
CP478_RS14240 2927266..2927697 + 432 WP_003899390.1 DUF4247 domain-containing protein -
CP478_RS14245 2927722..2928180 + 459 WP_003413451.1 DUF350 domain-containing protein -
CP478_RS14250 2928177..2929748 + 1572 WP_003899392.1 polyamine aminopropyltransferase -
CP478_RS14255 2929859..2930146 + 288 WP_003413456.1 antitoxin VapB41 Antitoxin
CP478_RS14260 2930133..2930573 + 441 WP_003413460.1 type II toxin-antitoxin system VapC family toxin Toxin
CP478_RS14265 2930594..2931349 - 756 WP_003413464.1 YebC/PmpR family DNA-binding transcriptional regulator -
CP478_RS14270 2931482..2932078 - 597 WP_003413465.1 pyridoxal 5'-phosphate synthase glutaminase subunit PdxT -
CP478_RS14275 2932086..2932931 - 846 WP_003413466.1 acyl-CoA thioesterase II -
CP478_RS14280 2932960..2933859 - 900 WP_003413468.1 pyridoxal 5'-phosphate synthase lyase subunit PdxS -
CP478_RS14285 2933987..2934661 + 675 WP_003413471.1 pyridoxamine 5'-phosphate oxidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-146)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 147 a.a.        Molecular weight: 16026.38 Da        Isoelectric Point: 6.8599

>T84690 WP_003413460.1 NZ_CP023622:2930133-2930573 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Download         Length: 441 bp

>T84690 NZ_CP023622:2930133-2930573 [Mycobacterium tuberculosis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10453.98 Da        Isoelectric Point: 7.2797

>AT84690 WP_003413456.1 NZ_CP023622:2929859-2930146 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGGAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Download         Length: 288 bp

>AT84690 NZ_CP023622:2929859-2930146 [Mycobacterium tuberculosis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQK0

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BWB8

References