Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 2869517..2870154 Replicon chromosome
Accession NZ_CP023622
Organism Mycobacterium tuberculosis strain MDRDM827

Toxin (Protein)


Gene name vapC Uniprot ID P0DMV7
Locus tag CP478_RS13975 Protein ID WP_003413180.1
Coordinates 2869517..2869912 (-) Length 132 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ44
Locus tag CP478_RS13980 Protein ID WP_003413183.1
Coordinates 2869909..2870154 (-) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP478_RS13930 2864920..2866131 + 1212 WP_003900845.1 alpha/beta hydrolase family protein -
CP478_RS13940 2866258..2866917 + 660 WP_003900846.1 LppA family lipoprotein -
CP478_RS13945 2866914..2867576 + 663 WP_003900848.1 hypothetical protein -
CP478_RS13950 2867573..2867851 + 279 WP_003901422.1 type II toxin-antitoxin system VapB family antitoxin -
CP478_RS13955 2867944..2868357 + 414 WP_003413164.1 PIN domain nuclease -
CP478_RS13960 2868396..2868653 + 258 WP_003413167.1 ribbon-helix-helix domain-containing protein -
CP478_RS13965 2868650..2869027 + 378 WP_003413174.1 type II toxin-antitoxin system VapC family toxin -
CP478_RS13970 2869043..2869417 - 375 WP_003413177.1 hypothetical protein -
CP478_RS13975 2869517..2869912 - 396 WP_003413180.1 type II toxin-antitoxin system toxin 23S rRNA-specific endonuclease VapC20 Toxin
CP478_RS13980 2869909..2870154 - 246 WP_003413183.1 type II toxin-antitoxin system antitoxin VapB20 Antitoxin
CP478_RS13990 2870565..2870984 - 420 WP_003413190.1 A24 family peptidase -
CP478_RS13995 2870996..2871805 - 810 WP_003413193.1 shikimate dehydrogenase -
CP478_RS14000 2871802..2873055 - 1254 WP_003413196.1 endolytic transglycosylase MltG -
CP478_RS14005 2873048..2873560 - 513 WP_003413197.1 Holliday junction resolvase RuvX -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Endonuclease VapC20-like

(1-129)

Antitoxin

Ribbon-helix-helix protein, CopG

(11-49)


Sequences

Toxin        


Download         Length: 132 a.a.        Molecular weight: 14619.53 Da        Isoelectric Point: 6.9794

>T84689 WP_003413180.1 NZ_CP023622:c2869912-2869517 [Mycobacterium tuberculosis]
MIFVDTSFWAALGNAGDARHGTAKRLWASKPPVVMTSNHVLGETWTLLNRRCGHRAAVAAAAIRLSTVVRVEHVTADLEE
QAWEWLVRHDEREYSFVDATSFAVMRKKGIQNAYAFDGDFSAAGFVEVRPE

Download         Length: 396 bp

>T84689 NZ_CP023622:c2869912-2869517 [Mycobacterium tuberculosis]
ATGATCTTCGTCGACACGTCCTTCTGGGCTGCACTTGGCAACGCCGGCGACGCTCGGCACGGCACCGCGAAGAGGTTGTG
GGCCAGCAAACCGCCCGTGGTGATGACCTCCAACCATGTCCTGGGCGAGACCTGGACGCTGCTCAACCGGCGCTGCGGTC
ACCGCGCGGCGGTTGCCGCCGCTGCAATCCGCTTGAGCACCGTCGTTCGCGTCGAGCACGTAACAGCCGACCTGGAAGAG
CAGGCATGGGAATGGCTTGTACGCCACGACGAGCGAGAGTACTCGTTCGTCGATGCCACGAGCTTCGCGGTGATGCGAAA
GAAAGGCATCCAAAATGCGTATGCCTTCGATGGTGACTTCAGCGCGGCCGGATTTGTCGAGGTAAGACCCGAGTAG

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 9098.33 Da        Isoelectric Point: 5.6007

>AT84689 WP_003413183.1 NZ_CP023622:c2870154-2869909 [Mycobacterium tuberculosis]
MLVAYICHVKRLQIYIDEDVDRALAVEARRRRTSKAALIREYVAEHLRQPGPDPVDAFVGSFVGEADLSASVDDVVYGKH
E

Download         Length: 246 bp

>AT84689 NZ_CP023622:c2870154-2869909 [Mycobacterium tuberculosis]
ATGCTAGTGGCGTATATTTGCCATGTGAAAAGGCTGCAGATCTACATCGACGAAGACGTTGACCGAGCGCTTGCTGTTGA
AGCGCGACGGCGGCGGACGTCGAAGGCGGCGCTGATCCGGGAGTACGTCGCTGAGCACCTCCGGCAGCCGGGCCCAGATC
CGGTCGATGCCTTCGTCGGATCGTTCGTGGGGGAAGCCGACTTGTCCGCGTCCGTCGACGACGTGGTTTACGGAAAGCAC
GAATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 5WZ4
PDB 5WZF

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BW31

References