Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 2257897..2258523 Replicon chromosome
Accession NZ_CP023622
Organism Mycobacterium tuberculosis strain MDRDM827

Toxin (Protein)


Gene name vapC Uniprot ID P64926
Locus tag CP478_RS10955 Protein ID WP_003410075.1
Coordinates 2258125..2258523 (+) Length 133 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID A0A806JLL8
Locus tag CP478_RS10950 Protein ID WP_003911750.1
Coordinates 2257897..2258124 (+) Length 76 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP478_RS10940 2255936..2256280 - 345 WP_003410065.1 ferredoxin family protein -
CP478_RS10945 2256469..2257638 - 1170 WP_003899126.1 ATP-binding protein -
CP478_RS10950 2257897..2258124 + 228 WP_003911750.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP478_RS10955 2258125..2258523 + 399 WP_003410075.1 PIN domain nuclease Toxin
CP478_RS10960 2258706..2259137 - 432 WP_003410078.1 MarR family winged helix-turn-helix transcriptional regulator -
CP478_RS10965 2259238..2259672 + 435 WP_003914358.1 DUF1398 domain-containing protein -
CP478_RS10980 2260109..2260288 - 180 Protein_2080 hypothetical protein -
CP478_RS10985 2260349..2260996 + 648 WP_071854215.1 transposase -
CP478_RS10990 2260950..2261540 + 591 WP_003899131.1 IS110 family transposase -
CP478_RS10995 2261668..2262924 - 1257 WP_003410095.1 HNH endonuclease -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-120)

Antitoxin

Bacterial antitoxin of type II TA system, VapB

(2-43)


Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 14731.02 Da        Isoelectric Point: 6.7067

>T84680 WP_003410075.1 NZ_CP023622:2258125-2258523 [Mycobacterium tuberculosis]
MIVDTSVWIAYLSTSESLASRWLADRIAADSTVIVPEVVMMELLIGKTDEDTAALRRRLLQRFAIEPLAPVRDAEDAAAI
HRRCRRGGDTVRSLIDCQVAAMALRIGVAVAHRDRDYEAIRTHCGLRTEPLF

Download         Length: 399 bp

>T84680 NZ_CP023622:2258125-2258523 [Mycobacterium tuberculosis]
ATGATCGTCGACACCTCGGTCTGGATCGCATATCTCTCCACGTCAGAGTCGTTGGCCAGTCGCTGGCTAGCCGATCGCAT
TGCCGCTGACTCGACGGTGATCGTGCCCGAGGTGGTGATGATGGAGCTGCTGATCGGTAAGACCGATGAGGACACCGCCG
CACTGCGCCGACGGCTCCTGCAGCGATTCGCTATCGAACCGCTGGCCCCGGTCCGCGACGCGGAAGATGCCGCCGCCATT
CACCGGCGCTGTCGTCGCGGCGGCGACACCGTACGCAGCCTGATCGATTGCCAGGTGGCCGCGATGGCGTTGCGGATCGG
GGTCGCCGTGGCGCATCGTGATCGCGACTACGAGGCGATCCGCACACATTGCGGACTACGCACCGAGCCGTTGTTCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8346.16 Da        Isoelectric Point: 4.1621

>AT84680 WP_003911750.1 NZ_CP023622:2257897-2258124 [Mycobacterium tuberculosis]
VSRTNIEIDDELVAAAQRMYRLDSKRSAVDLALRRLVGEPLGRDEALALQGSGFDFSNDEIESFSDTDRKLADES

Download         Length: 228 bp

>AT84680 NZ_CP023622:2257897-2258124 [Mycobacterium tuberculosis]
GTGTCACGGACCAACATCGAGATCGACGACGAACTCGTGGCCGCCGCACAGCGGATGTACCGACTCGATTCCAAGCGAAG
TGCCGTCGACCTCGCGCTGCGCCGGCTCGTGGGTGAACCGTTGGGCCGCGATGAGGCTTTGGCGCTGCAGGGCAGCGGTT
TCGACTTCAGCAACGATGAGATCGAATCGTTCTCGGATACGGACCGCAAGCTCGCCGACGAGTCGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4CHG

Antitoxin

Source ID Structure
AlphaFold DB A0A806JLL8

References