Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2225265..2225953 Replicon chromosome
Accession NZ_CP023622
Organism Mycobacterium tuberculosis strain MDRDM827

Toxin (Protein)


Gene name vapC Uniprot ID P0A653
Locus tag CP478_RS10785 Protein ID WP_003409958.1
Coordinates 2225265..2225684 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag CP478_RS10790 Protein ID WP_003409968.1
Coordinates 2225693..2225953 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP478_RS10765 2220760..2221608 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
CP478_RS10770 2221571..2223016 - 1446 WP_003899115.1 APC family permease -
CP478_RS10775 2223195..2223881 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
CP478_RS10780 2224072..2225040 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
CP478_RS10785 2225265..2225684 - 420 WP_003409958.1 type II toxin-antitoxin system VapC family toxin Toxin
CP478_RS10790 2225693..2225953 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP478_RS10795 2226096..2227772 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
CP478_RS10800 2227760..2228413 - 654 WP_003409976.1 cutinase Cfp21 -
CP478_RS10805 2228528..2228758 + 231 WP_003409980.1 DUF167 domain-containing protein -
CP478_RS10810 2228843..2229754 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
CP478_RS10815 2229863..2230462 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14724.89 Da        Isoelectric Point: 7.9535

>T84677 WP_003409958.1 NZ_CP023622:c2225684-2225265 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Download         Length: 420 bp

>T84677 NZ_CP023622:c2225684-2225265 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT84677 WP_003409968.1 NZ_CP023622:c2225953-2225693 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT84677 NZ_CP023622:c2225953-2225693 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A829C4H9

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References