Detailed information of TA system    

insolicoBioinformatically predicted

Overview


TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 1946897..1947510 Replicon chromosome
Accession NZ_CP023622
Organism Mycobacterium tuberculosis strain MDRDM827

Toxin (Protein)


Gene name vapC Uniprot ID P9WFA2
Locus tag CP478_RS09385 Protein ID WP_003408465.1
Coordinates 1946897..1947286 (-) Length 130 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ52
Locus tag CP478_RS09390 Protein ID WP_003408469.1
Coordinates 1947283..1947510 (-) Length 76 a.a.

Genomic Context


Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP478_RS09355 1942527..1943441 + 915 WP_003898985.1 3-hydroxyacyl-CoA dehydrogenase family protein -
CP478_RS09360 1943444..1944274 + 831 WP_003906661.1 cyclase family protein -
CP478_RS09365 1944274..1944623 + 350 Protein_1774 cupin domain-containing protein -
CP478_RS09370 1944676..1945494 + 819 WP_031697633.1 3-keto-5-aminohexanoate cleavage protein -
CP478_RS09375 1945508..1946287 + 780 WP_003408460.1 IclR family transcriptional regulator -
CP478_RS09385 1946897..1947286 - 390 WP_003408465.1 PIN domain-containing protein Toxin
CP478_RS09390 1947283..1947510 - 228 WP_003408469.1 antitoxin Antitoxin
CP478_RS09395 1947728..1949212 + 1485 WP_009937637.1 biotin carboxylase -
CP478_RS09400 1949209..1950456 + 1248 WP_003408476.1 serine hydrolase -
CP478_RS09405 1950499..1950918 - 420 WP_003408483.1 hypothetical protein -
CP478_RS09410 1950908..1951618 - 711 WP_003408486.1 winged helix-turn-helix transcriptional regulator -

Associated MGEs


MGE
detail
Similar
MGEs
Relative
position
MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains


Predicted by InterproScan

Toxin

(2-116)

Antitoxin


No domain identified.



Sequences


Toxin        


Download         Length: 130 a.a.        Molecular weight: 13817.98 Da        Isoelectric Point: 7.0612

>T84672 WP_003408465.1 NZ_CP023622:c1947286-1946897 [Mycobacterium tuberculosis]
VIVLDASAAVELMLTTPAGAAVARRLRGETVHAPAHFDVEVIGAIRQAVVRQLISDHEGLVVVVNFLSLPVRRWPLKPFT
QRAYQLRSTHTVADGAYVALAEGLGVPLITCDGRLAQSHGHNAEIELVA

Download         Length: 390 bp

>T84672 NZ_CP023622:c1947286-1946897 [Mycobacterium tuberculosis]
GTGATCGTGTTGGACGCCTCGGCGGCCGTCGAGCTCATGCTCACGACGCCGGCGGGTGCGGCGGTCGCCCGGCGCTTGCG
GGGCGAAACCGTGCACGCCCCTGCACATTTCGATGTCGAGGTGATCGGGGCGATACGTCAAGCGGTCGTGCGTCAGCTGA
TCAGCGATCATGAAGGACTCGTCGTGGTTGTCAACTTCCTTAGCCTGCCCGTTCGGCGTTGGCCGTTAAAGCCATTCACC
CAGCGTGCCTACCAACTTCGGTCCACTCATACCGTGGCCGATGGAGCCTACGTCGCCCTCGCCGAGGGCCTCGGCGTACC
GCTTATCACTTGCGACGGGCGACTTGCCCAATCCCATGGCCATAATGCGGAGATCGAGCTTGTCGCCTGA

Antitoxin


Download         Length: 76 a.a.        Molecular weight: 8226.39 Da        Isoelectric Point: 4.3313

>AT84672 WP_003408469.1 NZ_CP023622:c1947510-1947283 [Mycobacterium tuberculosis]
MSAMVQIRNVPDELLHELKARAAAQRMSLSDFLLARLAEIAEEPALDDVLDRLAALPRRDLGASAAELVDEARSE

Download         Length: 228 bp

>AT84672 NZ_CP023622:c1947510-1947283 [Mycobacterium tuberculosis]
ATGTCCGCCATGGTTCAGATCCGCAACGTTCCCGACGAGCTTCTCCACGAGCTGAAGGCCCGCGCAGCGGCGCAACGGAT
GAGCCTGAGCGATTTCCTCCTAGCACGGCTTGCCGAGATCGCGGAAGAACCGGCCCTCGACGACGTTCTCGACCGTCTAG
CGGCACTGCCGCGCCGGGATCTCGGCGCGAGCGCAGCCGAGCTTGTTGACGAGGCTCGATCTGAGTGA

Similar Proteins


Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures


Toxin

Source ID Structure
AlphaFold DB A0A7U4BUI9


Antitoxin

Source ID Structure
PDB 7E4J
AlphaFold DB A0A7U4FAN9

References