Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC-VapB
Location 752845..753478 Replicon chromosome
Accession NZ_CP023622
Organism Mycobacterium tuberculosis strain MDRDM827

Toxin (Protein)


Gene name vapC Uniprot ID G0TQD6
Locus tag CP478_RS03540 Protein ID WP_003403365.1
Coordinates 752845..753228 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ56
Locus tag CP478_RS03545 Protein ID WP_003403368.1
Coordinates 753323..753478 (-) Length 52 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP478_RS03515 748137..748673 + 537 WP_003403341.1 50S ribosomal protein L10 -
CP478_RS03520 748710..749102 + 393 WP_003403353.1 50S ribosomal protein L7/L12 -
CP478_RS03525 749095..749790 - 696 WP_003403355.1 TetR/AcrR family transcriptional regulator -
CP478_RS03530 749861..751366 + 1506 WP_003403360.1 carotenoid cleavage oxygenase -
CP478_RS03535 751447..752457 + 1011 WP_009937374.1 ABC transporter ATP-binding protein -
CP478_RS03540 752845..753228 - 384 WP_003403365.1 ribonuclease VapC6 Toxin
CP478_RS03545 753323..753478 - 156 WP_003403368.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP478_RS03550 753554..754270 - 717 WP_003403371.1 CPBP family intramembrane metalloprotease -
CP478_RS03555 754546..754854 - 309 WP_003403376.1 type II toxin-antitoxin system PemK/MazF family toxin -
CP478_RS03560 754841..755086 - 246 WP_003403381.1 ribbon-helix-helix protein, CopG family -
CP478_RS03565 755196..755633 - 438 WP_003403386.1 type II toxin-antitoxin system VapC family toxin -
CP478_RS03570 755630..755884 - 255 WP_003911263.1 antitoxin VapB7 -
CP478_RS03575 755998..758361 + 2364 WP_003898547.1 arylsulfatase AtsD -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Bacterial antitoxin of type II TA system, VapB

(3-44)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 13959.73 Da        Isoelectric Point: 6.0803

>T84656 WP_003403365.1 NZ_CP023622:c753228-752845 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLICDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Download         Length: 384 bp

>T84656 NZ_CP023622:c753228-752845 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGATCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin


Download         Length: 52 a.a.        Molecular weight: 5872.72 Da        Isoelectric Point: 5.7734

>AT84656 WP_003403368.1 NZ_CP023622:c753478-753323 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Download         Length: 156 bp

>AT84656 NZ_CP023622:c753478-753323 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB G0TQD6

Antitoxin

Source ID Structure
AlphaFold DB A0A7U4BSF8

References