Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 703105..703748 Replicon chromosome
Accession NZ_CP023622
Organism Mycobacterium tuberculosis strain MDRDM827

Toxin (Protein)


Gene name vapC Uniprot ID P67239
Locus tag CP478_RS03255 Protein ID WP_003403187.1
Coordinates 703347..703748 (+) Length 134 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TQ91
Locus tag CP478_RS03250 Protein ID WP_003403184.1
Coordinates 703105..703350 (+) Length 82 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP478_RS03215 698385..698915 - 531 WP_003917320.1 two component system sensor kinase HK2 -
CP478_RS03220 698899..699594 - 696 WP_003918099.1 two-component system response regulator TcrA -
CP478_RS03225 699717..700028 + 312 WP_003403164.1 hypothetical protein -
CP478_RS03230 700100..701050 + 951 WP_003900184.1 DUF1259 domain-containing protein -
CP478_RS03235 701291..701875 + 585 WP_003403171.1 IS607-like element IS1536 family transposase -
CP478_RS03240 701877..702587 + 711 Protein_626 transposase -
CP478_RS03245 702590..703060 + 471 WP_003898523.1 hypothetical protein -
CP478_RS03250 703105..703350 + 246 WP_003403184.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP478_RS03255 703347..703748 + 402 WP_003403187.1 type II toxin-antitoxin system VapC family toxin Toxin
CP478_RS21480 704136..704303 - 168 WP_077385191.1 DUF3800 domain-containing protein -
CP478_RS21485 704336..704533 - 198 WP_003403191.1 hypothetical protein -
CP478_RS03275 704613..705770 - 1158 WP_003898524.1 hypothetical protein -
CP478_RS03280 705822..706043 - 222 WP_003898525.1 DUF4926 domain-containing protein -
CP478_RS03285 706185..706790 + 606 WP_003898526.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-124)

Antitoxin

Antitoxin VapB-like

(1-80)


Sequences

Toxin        


Download         Length: 134 a.a.        Molecular weight: 14501.21 Da        Isoelectric Point: 4.4463

>T84652 WP_003403187.1 NZ_CP023622:703347-703748 [Mycobacterium tuberculosis]
VIVDTSAIIAILRDEDDAAAYADALANADVRRLSAASYLECGIVLDSQRDPVISRALDELIEEAEFVVEPVTERQARLAR
AAYADFGRGSGHPAGLNFGDCLSYALAIDRREPLLWKGNDFGHTGVQRALDRR

Download         Length: 402 bp

>T84652 NZ_CP023622:703347-703748 [Mycobacterium tuberculosis]
GTGATCGTCGACACGTCGGCGATCATCGCGATTCTGCGCGACGAGGACGACGCCGCGGCCTACGCCGACGCGCTCGCCAA
CGCCGATGTCCGCAGACTGTCTGCGGCCAGCTACCTGGAATGCGGGATAGTCCTTGACTCCCAGCGTGATCCGGTCATCA
GCAGAGCACTGGATGAACTTATCGAAGAAGCCGAGTTCGTCGTCGAGCCGGTAACCGAGCGCCAGGCCCGCCTGGCCCGA
GCGGCCTACGCGGATTTCGGCAGAGGCAGCGGCCACCCCGCGGGCTTGAATTTCGGCGACTGCCTGTCCTATGCACTGGC
GATCGATCGACGTGAGCCGCTGCTGTGGAAGGGCAACGACTTTGGGCACACCGGCGTCCAAAGGGCACTGGATCGGCGGT
GA

Antitoxin


Download         Length: 82 a.a.        Molecular weight: 8813.12 Da        Isoelectric Point: 10.3486

>AT84652 WP_003403184.1 NZ_CP023622:703105-703350 [Mycobacterium tuberculosis]
MALNIKDPSVHQAVKQIAKITGESQARAVATAVNERLARLRSDDLAARLLAIGHKTASRMSPEAKRLDHDALLYDERGLP
A

Download         Length: 246 bp

>AT84652 NZ_CP023622:703105-703350 [Mycobacterium tuberculosis]
ATGGCGTTGAACATCAAAGATCCGTCGGTTCACCAGGCGGTCAAGCAGATCGCGAAAATCACCGGCGAATCTCAGGCTCG
GGCGGTGGCGACCGCGGTGAACGAGCGTCTGGCCAGACTGCGCAGCGACGATCTCGCCGCCCGGCTCTTGGCTATCGGCC
ACAAGACCGCGAGCAGGATGAGCCCGGAAGCAAAGCGCCTCGACCACGATGCTCTGCTGTATGACGAGCGAGGGCTGCCG
GCGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BSD6

Antitoxin

Source ID Structure
AlphaFold DB G0TQ91

References