Detailed information of TA system TA084647

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pezAT/zeta-ParD
• Location: 444114..445125
• Replicon: chromosome
• Accession: NZ_CP023622
• Organism: Mycobacterium tuberculosis strain MDRDM827

Toxin (Protein)

• Gene name: pezT
• Uniprot ID: A0A655I6S7
• Locus tag: CP478_RS01955
• Protein ID: WP_003906352.1
• Coordinates: 444114..444707 (-)
• Length: 198 a.a.

Antitoxin (Protein)

• Gene name: pezA
• Uniprot ID: O53702
• Locus tag: CP478_RS01960
• Protein ID: WP_003401860.1
• Coordinates: 444736..445125 (-)
• Length: 130 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP478_RS01935	439763..441145	+	1383	WP_003898415.1	magnesium transporter	-
CP478_RS01940	441157..442191	-	1035	WP_003401856.1	class II fructose-bisphosphate aldolase	-
CP478_RS01945	442287..442970	+	684	WP_003401857.1	VTT domain-containing protein	-
CP478_RS01950	442959..444089	-	1131	WP_003401858.1	hypothetical protein	-
CP478_RS01955	444114..444707	-	594	WP_003906352.1	zeta toxin family protein	Toxin
CP478_RS01960	444736..445125	-	390	WP_003401860.1	hypothetical protein	Antitoxin
CP478_RS01965	445206..446417	-	1212	WP_003906353.1	VWA domain-containing protein	-
CP478_RS01970	446423..447025	-	603	WP_003898417.1	SRPBCC family protein	-
CP478_RS01975	447039..447935	-	897	WP_003898418.1	MoxR family ATPase	-
CP478_RS01980	447932..448525	-	594	WP_003401864.1	NTP transferase domain-containing protein	-
CP478_RS01985	448522..449277	-	756	WP_003401865.1	XdhC family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Zeta toxin domain (4-156)

Antitoxin

• ParD-like antitoxin of type II bacterial toxin-antitoxin system (6-106)

Sequences

Toxin

Protein

• Length: 198 a.a.
• Molecular weight: 21358.60 Da
• Isoelectric Point: 10.6337

>T84647 WP_003906352.1 NZ_CP023622:c444707-444114 [Mycobacterium tuberculosis]
VKRLDLVAGPNGAGKSTFVALTLAPLLPGIVFVNADEIAKQRWPDDPTSHAYQAAQVAADTRARLIDLGRPFIAETVFSH
PSKLELIRTARTAGYTVVLHVLVIPEGLAVERVRHRVAAGGHDVPETKIRERHRRLAELVAQAITLADGATVYDKSRLAG
PRIVAQFSGGGIIGRACWPSWTPPPLMSRWSNRPETA

Nucleotide

• Length: 594 bp

>T84647 NZ_CP023622:c444707-444114 [Mycobacterium tuberculosis]
GTGAAGCGGCTCGATCTGGTCGCCGGGCCCAACGGCGCCGGCAAGTCGACGTTCGTCGCCCTCACGCTGGCGCCCTTGCT
GCCCGGCATCGTCTTCGTCAACGCCGACGAAATCGCCAAACAACGCTGGCCCGACGACCCAACATCGCACGCCTACCAGG
CGGCGCAGGTCGCCGCCGACACCCGCGCGAGGCTCATCGACTTGGGCCGGCCGTTCATTGCCGAGACGGTGTTCTCGCAC
CCATCGAAGCTCGAGCTCATCCGCACCGCGCGCACGGCCGGCTACACCGTCGTACTGCACGTGTTGGTTATCCCCGAAGG
CCTGGCGGTCGAGCGCGTCAGGCATCGCGTCGCCGCGGGCGGCCACGATGTGCCGGAGACCAAGATCCGCGAGCGTCACC
GCCGGCTGGCCGAGCTTGTCGCCCAGGCCATCACGCTGGCCGACGGCGCCACCGTCTATGACAAAAGCCGGCTCGCGGGC
CCGCGGATCGTCGCTCAGTTCAGCGGGGGTGGCATCATCGGGCGCGCATGCTGGCCTTCGTGGACGCCGCCACCCTTGAT
GTCACGCTGGAGTAACAGGCCTGAGACGGCGTAA

Antitoxin

Protein

• Length: 130 a.a.
• Molecular weight: 13648.21 Da
• Isoelectric Point: 6.0829

>AT84647 WP_003401860.1 NZ_CP023622:c445125-444736 [Mycobacterium tuberculosis]
VPKAVDRVTRVAADLVDSAAAEGARQSRSAKQQLDHWARVGRAVSNQHTASRRRVEAALAGHLPMTDLTLEEGVVFNAEI
SAAIEERLSRTNYGDVLAAQGITTVALNDAGDIVEHRPDGTSVVLAATP

Nucleotide

• Length: 390 bp

>AT84647 NZ_CP023622:c445125-444736 [Mycobacterium tuberculosis]
GTGCCCAAAGCCGTCGACCGCGTCACGCGCGTCGCCGCGGATCTGGTGGACAGCGCGGCGGCCGAGGGTGCTCGTCAGAG
CCGTTCGGCCAAGCAGCAGCTCGACCACTGGGCCCGCGTCGGTCGGGCGGTGTCGAACCAGCACACGGCGTCGCGGCGCC
GCGTGGAAGCTGCGCTCGCGGGGCATCTGCCGATGACGGACCTGACGCTGGAGGAGGGCGTGGTGTTCAACGCCGAGATC
TCCGCAGCTATCGAGGAGCGCCTGTCGCGAACCAACTACGGCGACGTGCTCGCCGCGCAGGGCATCACCACGGTCGCCCT
GAACGACGCGGGCGACATCGTCGAGCACCGGCCAGACGGCACCTCGGTCGTGCTGGCGGCCACGCCGTGA

Structures

Toxin

• AlphaFold DB: A0A655I6S7

Antitoxin

• AlphaFold DB: A0A7U4BRT0