Detailed information of TA system TA084645

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): Rv0299-vapB/-
• Location: 363442..364013
• Replicon: chromosome
• Accession: NZ_CP023622
• Organism: Mycobacterium tuberculosis strain MDRDM827

Toxin (Protein)

• Gene name: Rv0299
• Uniprot ID: -
• Locus tag: CP478_RS01610
• Protein ID: WP_003401560.1
• Coordinates: 363442..363744 (+)
• Length: 101 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: O07227
• Locus tag: CP478_RS01615
• Protein ID: WP_003401563.1
• Coordinates: 363792..364013 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP478_RS01590	358911..359714	-	804	WP_003401540.1	hypothetical protein	-
CP478_RS01595	359724..361121	-	1398	WP_003401544.1	sulfatase	-
CP478_RS01600	361300..363075	+	1776	WP_010886074.1	PE family protein	-
CP478_RS01605	363218..363445	+	228	WP_003401555.1	ribbon-helix-helix protein, CopG family	-
CP478_RS01610	363442..363744	+	303	WP_003401560.1	toxin-antitoxin system toxin	Toxin
CP478_RS01615	363792..364013	+	222	WP_003401563.1	type II toxin-antitoxin system antitoxin VapB2	Antitoxin
CP478_RS01620	364010..364435	+	426	WP_003401566.1	PIN domain nuclease	-
CP478_RS01625	364571..365203	+	633	WP_003401571.1	TetR/AcrR family transcriptional regulator	-
CP478_RS01630	365200..366108	+	909	WP_003900117.1	SDR family NAD(P)-dependent oxidoreductase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Ribbon-helix-helix protein, CopG (9-37)

Sequences

Toxin

Protein

• Length: 101 a.a.
• Molecular weight: 10442.16 Da
• Isoelectric Point: 4.9609

>T84645 WP_003401560.1 NZ_CP023622:363442-363744 [Mycobacterium tuberculosis]
MIAPGDIAPRRDSEHELYVAVLSNALHRAADTGRVITCPFIPGRVPEDLLAMVVAVEQPNGTLLPELVQWLHVAALGAPL
GNAGVAALREAASVVTALLC

Nucleotide

• Length: 303 bp

>T84645 NZ_CP023622:363442-363744 [Mycobacterium tuberculosis]
TTGATCGCTCCCGGCGACATCGCGCCGCGCCGCGACAGTGAACACGAGCTCTACGTCGCCGTCTTGTCCAACGCGCTCCA
TCGGGCCGCGGACACCGGACGGGTGATCACCTGCCCATTCATTCCGGGCCGGGTCCCCGAGGATCTCTTGGCGATGGTGG
TGGCGGTCGAGCAACCCAACGGCACGCTGCTGCCGGAACTCGTGCAGTGGCTTCATGTTGCCGCGCTCGGTGCGCCACTC
GGCAACGCGGGCGTGGCCGCCCTACGCGAGGCTGCCTCGGTCGTGACAGCTCTGCTCTGTTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8088.31 Da
• Isoelectric Point: 10.7177

>AT84645 WP_003401563.1 NZ_CP023622:363792-364013 [Mycobacterium tuberculosis]
MSDVLIRDIPDDVLASLDAIAARLGLSRTEYIRRRLAQDAQTARVTVTAADLRRLRGAVAGLGDPELMRQAWR

Nucleotide

• Length: 222 bp

>AT84645 NZ_CP023622:363792-364013 [Mycobacterium tuberculosis]
ATGAGTGATGTACTGATTCGGGACATCCCCGACGACGTGTTAGCAAGCCTTGACGCGATCGCGGCACGCTTGGGCTTGTC
GCGGACCGAATACATCCGTCGGCGTTTAGCCCAGGATGCGCAGACGGCTCGCGTCACCGTGACAGCCGCGGATCTTCGAC
GCCTCAGGGGTGCGGTTGCCGGTCTGGGCGATCCCGAGCTTATGCGTCAGGCGTGGAGGTGA

Structures

Antitoxin

• PDB: 3H87
• AlphaFold DB: A0A829CG48