Detailed information of TA system TA084613

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mazEF/MazF(toxin)
• Location: 2194504..2195207
• Replicon: chromosome
• Accession: NZ_CP023621
• Organism: Mycobacterium tuberculosis strain LN2900

Toxin (Protein)

• Gene name: mazF
• Uniprot ID: -
• Locus tag: CP477_RS10555
• Protein ID: WP_096879789.1
• Coordinates: 2194504..2194833 (-)
• Length: 110 a.a.

Antitoxin (Protein)

• Gene name: mazE
• Uniprot ID: G0TLK8
• Locus tag: CP477_RS10560
• Protein ID: WP_003409780.1
• Coordinates: 2194830..2195207 (-)
• Length: 126 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP477_RS10535	2190887..2191957	+	1071	WP_003899091.1	epoxide hydrolase EphB	-
CP477_RS10540	2191954..2192469	+	516	WP_003409718.1	flavin reductase family protein	-
CP477_RS10545	2192466..2193527	+	1062	WP_003899092.1	3,4-dihydroxy-2-butanone-4-phosphate synthase	-
CP477_RS10550	2193524..2194294	+	771	WP_003409775.1	SDR family oxidoreductase	-
CP477_RS10555	2194504..2194833	-	330	WP_096879789.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
CP477_RS10560	2194830..2195207	-	378	WP_003409780.1	type II toxin-antitoxin system antitoxin MazE5	Antitoxin
CP477_RS10565	2195204..2195794	-	591	WP_003409784.1	SEC-C domain-containing protein	-
CP477_RS10570	2195849..2197213	+	1365	WP_003903691.1	HNH endonuclease	-
CP477_RS10575	2197368..2197820	-	453	WP_003899095.1	lipoprotein	-
CP477_RS10580	2197884..2198285	+	402	WP_003409869.1	hypothetical protein	-
CP477_RS10585	2198278..2198460	-	183	WP_003409870.1	hypothetical protein	-
CP477_RS10590	2198574..2198924	-	351	WP_003899096.1	hypothetical protein	-
CP477_RS10595	2198935..2199837	-	903	WP_003899097.1	hypothetical protein	-
CP477_RS10600	2199858..2200049	-	192	WP_003409876.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (3-106)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 110 a.a.
• Molecular weight: 11801.84 Da
• Isoelectric Point: 8.5361

>T84613 WP_096879789.1 NZ_CP023621:c2194833-2194504 [Mycobacterium tuberculosis]
VTALPARGEVWWCEMAEIGRRPVVVLSRDAAIPRLRRALVAPCTTTIRGLASEVVLEPGSDPIPRRSAVNLDSVESVSVA
VLLNRLGRLADIRMRAICTALEVAVDCSR

Nucleotide

• Length: 330 bp

>T84613 NZ_CP023621:c2194833-2194504 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGCTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 126 a.a.
• Molecular weight: 13560.07 Da
• Isoelectric Point: 5.1519

>AT84613 WP_003409780.1 NZ_CP023621:c2195207-2194830 [Mycobacterium tuberculosis]
VKTARLQVTLRCAVDLINSSSDQCFARIEHVASDQADPRPGVWHSSGMNRIRLSTTVDAALLTSARDMRAGITDAALIDE
ALAALLARHRSAEVDASYAAYDKHPVDEPDEWGDLASWRRAAGDS

Nucleotide

• Length: 378 bp

>AT84613 NZ_CP023621:c2195207-2194830 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Antitoxin

• AlphaFold DB: G0TLK8