Detailed information of TA system TA084509

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VapB
• Location: 3070108..3070787
• Replicon: chromosome
• Accession: NZ_CP023619
• Organism: Mycobacterium tuberculosis strain LN1100

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P9WF90
• Locus tag: CP475_RS15090
• Protein ID: WP_003414059.1
• Coordinates: 3070108..3070524 (-)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TF64
• Locus tag: CP475_RS15095
• Protein ID: WP_003414061.1
• Coordinates: 3070521..3070787 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP475_RS15070	3066160..3067062	-	903	WP_003900564.1	4-hydroxy-tetrahydrodipicolinate synthase	-
CP475_RS15075	3067131..3067883	-	753	WP_003899465.1	FAD-dependent thymidylate synthase	-
CP475_RS15080	3068127..3068402	-	276	WP_003414055.1	type I restriction endonuclease subunit S	-
CP475_RS15085	3068399..3070021	-	1623	WP_003414057.1	type I restriction-modification system subunit M	-
CP475_RS15090	3070108..3070524	-	417	WP_003414059.1	type II toxin-antitoxin system toxin ribonuclease C21	Toxin
CP475_RS15095	3070521..3070787	-	267	WP_003414061.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP475_RS15100	3070813..3071208	-	396	WP_003414064.1	type II toxin-antitoxin system VapC family toxin	-
CP475_RS15105	3071205..3071474	-	270	WP_003414066.1	type II toxin-antitoxin system VapB family antitoxin	-
CP475_RS15110	3071484..3072578	-	1095	WP_003900565.1	restriction endonuclease subunit S	-
CP475_RS15115	3072575..3072994	-	420	WP_003414070.1	winged helix-turn-helix domain-containing protein	-
CP475_RS15120	3073068..3073547	-	480	WP_003414073.1	dihydrofolate reductase	-
CP475_RS15125	3073618..3074418	-	801	WP_003911953.1	thymidylate synthase	-
CP475_RS15130	3074574..3075311	+	738	WP_003414079.1	dienelactone hydrolase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-123)

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (16-56)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15773.00 Da
• Isoelectric Point: 7.1294

>T84509 WP_003414059.1 NZ_CP023619:c3070524-3070108 [Mycobacterium tuberculosis]
MTTRYLLDKSAAYRAHLPAVRHRLEPLMERGLLARCGITDLEFGVSARSREDHRTLGTYRRDALEYVNTPDTVWVRAWEI
QEALTDKGFHRSVKIPDLIIAAVAEHHGIPVMHYDQDFERIAAITRQPVEWVVAPGTA

Nucleotide

• Length: 417 bp

>T84509 NZ_CP023619:c3070524-3070108 [Mycobacterium tuberculosis]
ATGACCACGCGCTATTTGCTCGACAAATCAGCGGCTTACCGCGCGCACTTGCCCGCGGTCCGACATCGCTTGGAACCGTT
GATGGAACGCGGTCTACTGGCCCGGTGCGGCATTACCGATCTCGAGTTCGGAGTCTCGGCGCGTTCCCGCGAGGACCATC
GAACACTGGGCACCTACCGGCGTGACGCGCTCGAATACGTCAACACCCCCGACACCGTGTGGGTTCGTGCATGGGAGATC
CAAGAAGCATTGACCGACAAGGGATTTCACCGCTCGGTCAAGATCCCGGACTTGATCATTGCGGCGGTCGCCGAGCATCA
CGGCATACCCGTCATGCACTACGACCAAGACTTCGAACGCATCGCCGCCATCACACGGCAACCCGTGGAATGGGTGGTGG
CCCCAGGGACAGCGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9493.77 Da
• Isoelectric Point: 7.2783

>AT84509 WP_003414061.1 NZ_CP023619:c3070787-3070521 [Mycobacterium tuberculosis]
MHRGYALVVCSPGVTRTMIDIDDDLLARAAKELGTTTKKDTVHAALRAALRASAARSLMNRMAENATGTQDEALVNAMWR
DGHPENTA

Nucleotide

• Length: 267 bp

>AT84509 NZ_CP023619:c3070787-3070521 [Mycobacterium tuberculosis]
ATGCATCGCGGATATGCATTAGTAGTATGCTCTCCGGGTGTGACGCGCACCATGATCGACATCGATGACGACCTGCTCGC
TCGGGCCGCCAAGGAACTGGGTACCACGACAAAGAAAGACACCGTGCATGCCGCGCTGCGGGCCGCGTTGCGAGCCAGTG
CCGCGCGCTCGCTCATGAACCGGATGGCCGAGAACGCCACCGGAACCCAGGACGAAGCTCTGGTCAACGCAATGTGGCGA
GACGGCCACCCAGAGAACACGGCATGA

Structures

Toxin

• PDB: 5SV2

Antitoxin

• AlphaFold DB: G0TF64