Detailed information of TA system TA084502

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2808022..2808674
• Replicon: chromosome
• Accession: NZ_CP023619
• Organism: Mycobacterium tuberculosis strain LN1100

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CP475_RS13625
• Protein ID: WP_096865713.1
• Coordinates: 2808249..2808674 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ24
• Locus tag: CP475_RS13620
• Protein ID: WP_003412749.1
• Coordinates: 2808022..2808243 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP475_RS13605	2806262..2806468	-	207	WP_003899347.1	hypothetical protein	-
CP475_RS13610	2806604..2807227	+	624	WP_003900858.1	TIGR00725 family protein	-
CP475_RS13615	2807217..2807969	+	753	WP_003900859.1	hypothetical protein	-
CP475_RS13620	2808022..2808243	+	222	WP_003412749.1	antitoxin	Antitoxin
CP475_RS13625	2808249..2808674	+	426	WP_096865713.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP475_RS13630	2808697..2809878	-	1182	WP_003899351.1	2-oxo acid dehydrogenase subunit E2	-
CP475_RS13635	2809875..2810921	-	1047	WP_003412757.1	3-methyl-2-oxobutanoate dehydrogenase subunit beta	-
CP475_RS13640	2810932..2812035	-	1104	WP_003412761.1	pyruvate dehydrogenase (acetyl-transferring) E1 component subunit alpha	-
CP475_RS13645	2812294..2813115	-	822	WP_003412766.1	citrate (pro-3S)-lyase subunit beta	-
CP475_RS13650	2813112..2813669	-	558	WP_003412768.1	MaoC family dehydratase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-139)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15314.78 Da
• Isoelectric Point: 7.4767

>T84502 WP_096865713.1 NZ_CP023619:2808249-2808674 [Mycobacterium tuberculosis]
VALLDVNALVALAWDSHIHHARIREWFTANATLGWATCPLTEAGFVRVSTNPKVLPSAIGIADARPVLVALRAVGGHHFL
ADDVSLVDDDVPLIVGYRQVTDAHLLTLARRRGVRLVTFDAGVFTLAQQRPKTPVELLTIL

Nucleotide

• Length: 426 bp

>T84502 NZ_CP023619:2808249-2808674 [Mycobacterium tuberculosis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACCGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCACTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 7864.93 Da
• Isoelectric Point: 4.5258

>AT84502 WP_003412749.1 NZ_CP023619:2808022-2808243 [Mycobacterium tuberculosis]
VRTTLDLDDDVIAAARELASSQRRSLGSVISELARRGLMPGRVEADDGLPVIRVPAGTPPITPEMVRRALDED

Nucleotide

• Length: 222 bp

>AT84502 NZ_CP023619:2808022-2808243 [Mycobacterium tuberculosis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Structures

Antitoxin

• AlphaFold DB: A0A7U4BW03