Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/VapC(toxin)
Location 2225366..2226054 Replicon chromosome
Accession NZ_CP023619
Organism Mycobacterium tuberculosis strain LN1100

Toxin (Protein)


Gene name vapC Uniprot ID P9WF64
Locus tag CP475_RS10770 Protein ID WP_003899117.1
Coordinates 2225366..2225785 (-) Length 140 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID P9WJ28
Locus tag CP475_RS10775 Protein ID WP_003409968.1
Coordinates 2225794..2226054 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP475_RS10750 2220861..2221709 + 849 WP_003409942.1 class I SAM-dependent methyltransferase -
CP475_RS10755 2221672..2223117 - 1446 WP_003899115.1 APC family permease -
CP475_RS10760 2223296..2223982 - 687 WP_003409954.1 immunoprotective protein Mpt64 -
CP475_RS10765 2224173..2225141 - 969 WP_003899116.1 class 1b ribonucleoside-diphosphate reductase subunit beta -
CP475_RS10770 2225366..2225785 - 420 WP_003899117.1 type II toxin-antitoxin system VapC family toxin Toxin
CP475_RS10775 2225794..2226054 - 261 WP_003409968.1 type II toxin-antitoxin system VapB family antitoxin Antitoxin
CP475_RS10780 2226197..2227873 + 1677 WP_003899118.1 PecA family PE domain-processing aspartic protease -
CP475_RS10785 2227861..2228514 - 654 WP_003409976.1 cutinase Cfp21 -
CP475_RS10790 2228629..2228859 + 231 WP_003409980.1 DUF167 domain-containing protein -
CP475_RS10795 2228944..2229855 - 912 WP_003409986.1 ArgP/LysG family DNA-binding transcriptional regulator -
CP475_RS10800 2229964..2230563 + 600 WP_003409989.1 L-lysine exporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(1-125)

Antitoxin

Antitoxin VapB-like

(2-81)


Sequences

Toxin        


Download         Length: 140 a.a.        Molecular weight: 14748.87 Da        Isoelectric Point: 7.9722

>T84494 WP_003899117.1 NZ_CP023619:c2225785-2225366 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDHEFRGVVGYWPGVA

Download         Length: 420 bp

>T84494 NZ_CP023619:c2225785-2225366 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCATGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9528.88 Da        Isoelectric Point: 5.1007

>AT84494 WP_003409968.1 NZ_CP023619:c2226054-2225794 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Download         Length: 261 bp

>AT84494 NZ_CP023619:c2226054-2225794 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E7TLI0

Antitoxin

Source ID Structure
AlphaFold DB A0A829C483

References