Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/PIN(toxin)
Location 3182947..3183634 Replicon chromosome
Accession NZ_CP023611
Organism Mycobacterium tuberculosis strain LN763

Toxin (Protein)


Gene name vapC Uniprot ID P65044
Locus tag CP467_RS15690 Protein ID WP_003414624.1
Coordinates 3183191..3183634 (+) Length 148 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID G0TFH0
Locus tag CP467_RS15685 Protein ID WP_003414620.1
Coordinates 3182947..3183204 (+) Length 86 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP467_RS15665 3178267..3179121 - 855 WP_003899516.1 GNAT family N-acetyltransferase -
CP467_RS15670 3179177..3180340 - 1164 WP_003899517.1 flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase -
CP467_RS15675 3180357..3181571 - 1215 WP_003899518.1 zinc metalloprotease Rip -
CP467_RS15680 3181579..3182820 - 1242 WP_003414613.1 1-deoxy-D-xylulose-5-phosphate reductoisomerase -
CP467_RS15685 3182947..3183204 + 258 WP_003414620.1 ribbon-helix-helix domain-containing protein Antitoxin
CP467_RS15690 3183191..3183634 + 444 WP_003414624.1 type II toxin-antitoxin system VapC family toxin Toxin
CP467_RS15695 3183714..3184376 + 663 WP_003414630.1 cell surface glycolipoprotein Mpt83 -
CP467_RS15700 3184472..3184663 + 192 WP_003414632.1 type II toxin-antitoxin system Phd/YefM family antitoxin -
CP467_RS15705 3184995..3186743 + 1749 WP_003912869.1 cytochrome c biogenesis protein DipZ -
CP467_RS15710 3186839..3187420 + 582 WP_003414644.1 fasciclin domain-containing protein -
CP467_RS15715 3187520..3187786 + 267 WP_015456449.1 DUF2631 domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain toxin

(1-142)

Antitoxin

Ribbon-helix-helix protein, CopG

(2-38)


Sequences

Toxin        


Download         Length: 148 a.a.        Molecular weight: 16595.72 Da        Isoelectric Point: 6.4890

>T84027 WP_003414624.1 NZ_CP023611:3183191-3183634 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Download         Length: 444 bp

>T84027 NZ_CP023611:3183191-3183634 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin


Download         Length: 86 a.a.        Molecular weight: 9013.22 Da        Isoelectric Point: 10.8344

>AT84027 WP_003414620.1 NZ_CP023611:3182947-3183204 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Download         Length: 258 bp

>AT84027 NZ_CP023611:3182947-3183204 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 7VWO

Antitoxin

Source ID Structure
PDB 7VWO
AlphaFold DB G0TFH0

References