Detailed information of TA system TA083966

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 3183013..3183700
• Replicon: chromosome
• Accession: NZ_CP023610
• Organism: Mycobacterium tuberculosis strain LN317

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P65044
• Locus tag: CP466_RS15695
• Protein ID: WP_003414624.1
• Coordinates: 3183257..3183700 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: G0TFH0
• Locus tag: CP466_RS15690
• Protein ID: WP_003414620.1
• Coordinates: 3183013..3183270 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP466_RS15670	3178333..3179187	-	855	WP_003899516.1	GNAT family N-acetyltransferase	-
CP466_RS15675	3179243..3180406	-	1164	WP_003899517.1	flavodoxin-dependent (E)-4-hydroxy-3-methylbut-2-enyl-diphosphate synthase	-
CP466_RS15680	3180423..3181637	-	1215	WP_003899518.1	zinc metalloprotease Rip	-
CP466_RS15685	3181645..3182886	-	1242	WP_096879595.1	1-deoxy-D-xylulose-5-phosphate reductoisomerase	-
CP466_RS15690	3183013..3183270	+	258	WP_003414620.1	ribbon-helix-helix domain-containing protein	Antitoxin
CP466_RS15695	3183257..3183700	+	444	WP_003414624.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP466_RS15700	3183780..3184442	+	663	WP_003414630.1	cell surface glycolipoprotein Mpt83	-
CP466_RS15705	3184538..3184729	+	192	WP_096879460.1	type II toxin-antitoxin system prevent-host-death family antitoxin	-
CP466_RS15710	3185061..3186809	+	1749	WP_003912869.1	cytochrome c biogenesis protein DipZ	-
CP466_RS15715	3186905..3187486	+	582	WP_003414644.1	fasciclin domain-containing protein	-
CP466_RS15720	3187586..3187852	+	267	WP_015456449.1	DUF2631 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-142)

Antitoxin

• Ribbon-helix-helix protein, CopG (2-38)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16595.72 Da
• Isoelectric Point: 6.4890

>T83966 WP_003414624.1 NZ_CP023610:3183257-3183700 [Mycobacterium tuberculosis]
MLCVDVNVLVYAHRADLREHADYRGLLERLANDDEPLGLPDSVLAGFIRVVTNRRVFTEPTSPQDAWQAVDALLAAPAAM
RLRPGERHWMAFRQLASDVDANGNDIADAHLAAYALENNATWLSADRGFARFRRLRWRHPLDGQTHL

Nucleotide

• Length: 444 bp

>T83966 NZ_CP023610:3183257-3183700 [Mycobacterium tuberculosis]
ATGCTGTGCGTTGATGTCAACGTGCTCGTTTACGCGCATCGGGCAGACCTACGGGAGCACGCGGACTATCGGGGTTTGCT
TGAGCGGCTGGCCAACGATGACGAGCCGCTGGGTCTACCAGATAGCGTGCTCGCCGGCTTCATCCGGGTGGTTACCAACC
GCCGCGTCTTCACCGAGCCGACGAGCCCACAGGACGCATGGCAGGCAGTCGACGCCCTACTCGCGGCACCCGCAGCCATG
CGACTTCGGCCTGGCGAGCGCCACTGGATGGCCTTTCGGCAGTTAGCGTCCGATGTTGATGCGAACGGCAACGACATTGC
GGACGCGCACCTGGCCGCCTACGCGCTAGAGAACAACGCAACCTGGTTGAGCGCCGACCGCGGCTTTGCCCGTTTCCGTC
GACTGCGCTGGCGTCATCCGTTGGACGGTCAGACCCATCTATAA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9013.22 Da
• Isoelectric Point: 10.8344

>AT83966 WP_003414620.1 NZ_CP023610:3183013-3183270 [Mycobacterium tuberculosis]
VRTTIRIDDELYREVKAKAARSGRTVAAVLEDAVRRGLNPPKPQAAGRYRVQPSGKGGLRPGVDLSSNAALAEAMNDGVS
VDAVR

Nucleotide

• Length: 258 bp

>AT83966 NZ_CP023610:3183013-3183270 [Mycobacterium tuberculosis]
GTGCGCACGACGATCCGTATCGATGACGAGCTGTACCGCGAGGTGAAAGCAAAGGCCGCTCGTTCCGGGCGTACCGTGGC
CGCGGTTCTTGAAGATGCGGTGCGGCGTGGTCTCAACCCGCCTAAGCCGCAGGCCGCCGGCCGTTATCGAGTCCAGCCGT
CGGGTAAGGGCGGCCTGCGGCCCGGTGTCGATCTATCGTCCAACGCCGCACTTGCCGAAGCGATGAACGACGGCGTGTCG
GTCGATGCTGTGCGTTGA

Structures

Toxin

• PDB: 7VWO

Antitoxin

• AlphaFold DB: G0TFH0