Detailed information of TA system TA083964

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN_Sll0205-like-Phd
• Location: 3136497..3137101
• Replicon: chromosome
• Accession: NZ_CP023610
• Organism: Mycobacterium tuberculosis strain LN317

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: P71623
• Locus tag: CP466_RS15470
• Protein ID: WP_003414492.1
• Coordinates: 3136497..3136889 (-)
• Length: 131 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A829CBY8
• Locus tag: CP466_RS15475
• Protein ID: WP_003414495.1
• Coordinates: 3136886..3137101 (-)
• Length: 72 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP466_RS15440	3131647..3132435	-	789	WP_198403645.1	CRISPR-associated endoribonuclease Cas6	-
CP466_RS15445	3132769..3133314	-	546	WP_003913758.1	DUF1802 family protein	-
CP466_RS15450	3133586..3134470	-	885	WP_003414409.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
CP466_RS15455	3134473..3135360	-	888	WP_003414414.1	type IV toxin-antitoxin system AbiEi family antitoxin	-
CP466_RS15460	3135665..3136210	-	546	WP_003899500.1	DUF1802 family protein	-
CP466_RS15465	3136207..3136476	-	270	WP_003414489.1	DUF2277 family protein	-
CP466_RS15470	3136497..3136889	-	393	WP_003414492.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP466_RS15475	3136886..3137101	-	216	WP_003414495.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
CP466_RS15480	3137148..3137897	+	750	WP_003414497.1	enoyl-CoA hydratase	-
CP466_RS15485	3137976..3139058	-	1083	WP_096879455.1	ABC transporter ATP-binding protein	-
CP466_RS15490	3139051..3140361	-	1311	WP_003414501.1	ABC transporter substrate-binding protein	-
CP466_RS15495	3140364..3141191	-	828	WP_096879456.1	carbohydrate ABC transporter permease	-
CP466_RS15500	3141188..3142099	-	912	WP_003414505.1	sugar ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• VapC-like Sll0205 protein, PIN domain (5-130)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (2-46)

Sequences

Toxin

Protein

• Length: 131 a.a.
• Molecular weight: 14610.74 Da
• Isoelectric Point: 6.7594

>T83964 WP_003414492.1 NZ_CP023610:c3136889-3136497 [Mycobacterium tuberculosis]
MTTVLLDSHVAYWWSAEPQRLSMAASQAIEHADELAVAAISWFELAWLAEQERIQLAIPVLSWLQQLAEHVRTVGITPSV
AATAVALPSSFPGDPADRLIYATAIEHGWRLVTKDRRLRSHRHPRPVTVW

Nucleotide

• Length: 393 bp

>T83964 NZ_CP023610:c3136889-3136497 [Mycobacterium tuberculosis]
ATGACGACGGTGCTGCTCGACTCGCATGTGGCCTACTGGTGGTCGGCCGAGCCGCAGCGTCTCAGCATGGCGGCGAGCCA
GGCCATCGAACACGCCGACGAGCTCGCCGTCGCCGCGATTTCGTGGTTCGAGCTGGCTTGGCTTGCCGAACAGGAACGCA
TCCAACTGGCGATTCCGGTGCTGTCCTGGCTTCAGCAGCTGGCCGAGCACGTTCGCACCGTCGGTATCACGCCCTCGGTC
GCCGCCACGGCGGTGGCGCTGCCCTCGTCGTTCCCCGGCGATCCGGCCGACCGGTTGATCTACGCCACCGCGATCGAACA
CGGCTGGCGGCTGGTGACCAAGGACCGGCGGCTACGCAGTCATCGGCACCCACGACCGGTCACCGTCTGGTAG

Antitoxin

Protein

• Length: 72 a.a.
• Molecular weight: 7511.55 Da
• Isoelectric Point: 4.9732

>AT83964 WP_003414495.1 NZ_CP023610:c3137101-3136886 [Mycobacterium tuberculosis]
MTATEVKAKILSLLDEVAQGEEIEITKHGRTVARLVAATGPHALKGRFSGVAMAAVDDDELFTTGVSWNVS

Nucleotide

• Length: 216 bp

>AT83964 NZ_CP023610:c3137101-3136886 [Mycobacterium tuberculosis]
ATGACCGCTACGGAGGTGAAGGCGAAGATCCTCTCCTTGCTTGATGAAGTGGCCCAGGGCGAGGAGATCGAGATCACCAA
ACACGGCCGCACCGTGGCCCGGCTGGTGGCAGCGACGGGGCCGCACGCGCTGAAGGGTCGATTCTCGGGTGTGGCGATGG
CGGCCGTGGATGACGACGAACTCTTCACCACCGGGGTTTCGTGGAACGTTTCATGA

Structures

Toxin

• AlphaFold DB: A0A7U4BWM8

Antitoxin

• AlphaFold DB: A0A829CBY8