Detailed information of TA system TA083945

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC(toxin)
• Location: 2225290..2225978
• Replicon: chromosome
• Accession: NZ_CP023610
• Organism: Mycobacterium tuberculosis strain LN317

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CP466_RS10780
• Protein ID: WP_096872165.1
• Coordinates: 2225290..2225709 (-)
• Length: 140 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ28
• Locus tag: CP466_RS10785
• Protein ID: WP_003409968.1
• Coordinates: 2225718..2225978 (-)
• Length: 87 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP466_RS10760	2220785..2221633	+	849	WP_003409942.1	class I SAM-dependent methyltransferase	-
CP466_RS10765	2221596..2223041	-	1446	WP_003899115.1	APC family permease	-
CP466_RS10770	2223220..2223906	-	687	WP_096879404.1	immunoprotective protein Mpt64	-
CP466_RS10775	2224097..2225065	-	969	WP_003899116.1	class 1b ribonucleoside-diphosphate reductase subunit beta	-
CP466_RS10780	2225290..2225709	-	420	WP_096872165.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP466_RS10785	2225718..2225978	-	261	WP_003409968.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP466_RS10790	2226121..2227797	+	1677	WP_003899118.1	PecA family PE domain-processing aspartic protease	-
CP466_RS10795	2227785..2228438	-	654	WP_003409976.1	cutinase Cfp21	-
CP466_RS10800	2228553..2228783	+	231	WP_003409980.1	DUF167 domain-containing protein	-
CP466_RS10805	2228868..2229779	-	912	WP_003409986.1	ArgP/LysG family DNA-binding transcriptional regulator	-
CP466_RS10810	2229888..2230487	+	600	WP_003409989.1	L-lysine exporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (1-125)

Antitoxin

• Antitoxin VapB-like (2-81)

Sequences

Toxin

Protein

• Length: 140 a.a.
• Molecular weight: 14724.89 Da
• Isoelectric Point: 7.9535

>T83945 WP_096872165.1 NZ_CP023610:c2225709-2225290 [Mycobacterium tuberculosis]
MIVDTSAVVALVQGERPHATLVAAALAGAHSPVMSAPTVAECLIVLTARHGPVARTIFERLRSEIGLSVSSFTAEHAAAT
QRAFLRYGKGRHRAALNFGDCMTYATAQLGHQPLLAVGNDFPQTDLEFRGVVGYWPGVA

Nucleotide

• Length: 420 bp

>T83945 NZ_CP023610:c2225709-2225290 [Mycobacterium tuberculosis]
ATGATCGTGGACACAAGCGCCGTGGTGGCCCTGGTTCAAGGCGAGCGGCCGCACGCCACCCTGGTCGCGGCCGCCCTGGC
CGGCGCCCATAGCCCCGTCATGTCTGCACCCACCGTCGCCGAATGCCTGATTGTCTTGACCGCCCGTCACGGCCCCGTTG
CGCGCACGATCTTCGAACGACTTCGCAGCGAAATCGGCTTGAGCGTGTCATCTTTCACCGCCGAGCATGCCGCTGCCACG
CAACGAGCCTTTCTGCGATACGGCAAGGGGCGCCACCGCGCGGCTCTCAACTTCGGAGACTGTATGACGTACGCGACCGC
CCAGCTGGGCCACCAACCACTGCTGGCCGTCGGCAACGACTTCCCGCAAACCGACCTTGAGTTCCGCGGCGTCGTCGGCT
ACTGGCCAGGCGTCGCGTAA

Antitoxin

Protein

• Length: 87 a.a.
• Molecular weight: 9528.88 Da
• Isoelectric Point: 5.1007

>AT83945 WP_003409968.1 NZ_CP023610:c2225978-2225718 [Mycobacterium tuberculosis]
VALNIKDPEVDRLAAELADRLHTSKTAAIRHALSAQLAFLESRAGDREAQLLDILRTEIWPLLADRSPITKLEREQILGY
DPATGV

Nucleotide

• Length: 261 bp

>AT83945 NZ_CP023610:c2225978-2225718 [Mycobacterium tuberculosis]
GTGGCGCTGAATATCAAAGACCCTGAGGTAGACCGACTAGCCGCCGAACTCGCTGACCGGCTGCACACCAGCAAGACTGC
CGCCATCCGGCATGCCCTGTCTGCCCAGCTGGCGTTTTTGGAGTCGCGCGCCGGCGACCGTGAGGCACAACTTCTCGACA
TCTTGCGTACCGAAATCTGGCCCCTGCTTGCCGACCGCTCCCCCATCACCAAGCTCGAGCGCGAACAAATCCTCGGCTAC
GACCCCGCAACCGGAGTCTGA

Structures

Antitoxin

• AlphaFold DB: A0A829C483