Detailed information of TA system TA083924

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapB(antitoxin)
• Location: 752866..753499
• Replicon: chromosome
• Accession: NZ_CP023610
• Organism: Mycobacterium tuberculosis strain LN317

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: CP466_RS03545
• Protein ID: WP_096879308.1
• Coordinates: 752866..753249 (-)
• Length: 128 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: P9WJ56
• Locus tag: CP466_RS03550
• Protein ID: WP_003403368.1
• Coordinates: 753344..753499 (-)
• Length: 52 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP466_RS03520	748158..748694	+	537	WP_003403341.1	50S ribosomal protein L10	-
CP466_RS03525	748731..749123	+	393	WP_003403353.1	50S ribosomal protein L7/L12	-
CP466_RS03530	749116..749811	-	696	WP_003403355.1	TetR/AcrR family transcriptional regulator	-
CP466_RS03535	749882..751387	+	1506	WP_003403360.1	carotenoid cleavage oxygenase	-
CP466_RS03540	751468..752478	+	1011	WP_003911248.1	ABC transporter ATP-binding protein	-
CP466_RS03545	752866..753249	-	384	WP_096879308.1	VapC toxin family PIN domain ribonuclease	Toxin
CP466_RS03550	753344..753499	-	156	WP_003403368.1	type II toxin-antitoxin system VapB family antitoxin	Antitoxin
CP466_RS03555	753575..754291	-	717	WP_003403371.1	CPBP family intramembrane metalloprotease	-
CP466_RS03560	754567..754875	-	309	WP_003403376.1	type II toxin-antitoxin system PemK/MazF family toxin	-
CP466_RS03565	754862..755107	-	246	WP_003403381.1	ribbon-helix-helix protein, CopG family	-
CP466_RS03570	755217..755654	-	438	WP_003403386.1	type II toxin-antitoxin system VapC family toxin	-
CP466_RS03575	755651..755905	-	255	WP_003911263.1	antitoxin VapB7	-
CP466_RS03580	756019..758382	+	2364	WP_003898547.1	arylsulfatase AtsD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Bacterial antitoxin of type II TA system, VapB (3-44)

Sequences

Toxin

Protein

• Length: 128 a.a.
• Molecular weight: 13945.70 Da
• Isoelectric Point: 6.0803

>T83924 WP_096879308.1 NZ_CP023610:c753249-752866 [Mycobacterium tuberculosis]
MAAATTTGTHRGLELRAAQRAVGSCEPQRAEFCRSARNADEFDQMSRMFGDVYPDVPVPKSVWRWIDSAQHRLARAGAVG
ALSVVDLLVCDTAAARGLVVLHDDADYELAERHLPDIRVRRVVSADD

Nucleotide

• Length: 384 bp

>T83924 NZ_CP023610:c753249-752866 [Mycobacterium tuberculosis]
TTGGCGGCTGCAACGACAACCGGAACCCACCGAGGCCTGGAACTCCGCGCTGCTCAGCGGGCCGTCGGGTCGTGCGAACC
GCAACGAGCCGAGTTCTGCCGATCAGCGCGGAATGCGGACGAGTTCGACCAGATGAGCCGGATGTTTGGTGACGTCTACC
CCGATGTGCCAGTGCCGAAATCCGTGTGGCGGTGGATCGATTCGGCACAGCACCGCCTCGCCCGGGCGGGAGCGGTGGGT
GCCCTGTCGGTTGTCGATCTGCTGGTCTGCGACACTGCGGCGGCCAGGGGCCTAGTAGTCCTCCACGACGATGCCGACTA
CGAACTCGCAGAGCGTCACCTTCCCGACATCAGAGTGCGGAGGGTCGTCAGCGCCGACGACTAG

Antitoxin

Protein

• Length: 52 a.a.
• Molecular weight: 5872.72 Da
• Isoelectric Point: 5.7734

>AT83924 WP_003403368.1 NZ_CP023610:c753499-753344 [Mycobacterium tuberculosis]
VSVTQIDLDDEALADVMRIAAVHTKKEAVNLAMRDYVERFRRIEALARSRE

Nucleotide

• Length: 156 bp

>AT83924 NZ_CP023610:c753499-753344 [Mycobacterium tuberculosis]
GTGTCGGTGACCCAGATCGACCTCGACGACGAAGCGCTGGCCGATGTGATGCGCATCGCCGCCGTGCACACAAAGAAGGA
AGCCGTGAACCTCGCGATGCGCGATTACGTCGAACGGTTTCGGCGCATCGAGGCCCTGGCCCGCTCGCGCGAGTAG

Structures

Antitoxin

• AlphaFold DB: A0A7U4BSF8