Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) mazEF/MazF(toxin)
Location 1230546..1231093 Replicon chromosome
Accession NZ_CP023609
Organism Mycobacterium tuberculosis strain LN55

Toxin (Protein)


Gene name mazF Uniprot ID A0A7U4F9D0
Locus tag CP465_RS06045 Protein ID WP_003405820.1
Coordinates 1230546..1230857 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name mazE Uniprot ID Q8VK70
Locus tag CP465_RS06050 Protein ID WP_003913656.1
Coordinates 1230857..1231093 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
CP465_RS06025 1226027..1227451 - 1425 WP_003405805.1 class II fumarate hydratase -
CP465_RS06030 1227482..1228570 - 1089 WP_003898726.1 class II fructose-bisphosphatase -
CP465_RS06035 1228626..1229270 + 645 WP_003915494.1 DUF4245 domain-containing protein -
CP465_RS06040 1229277..1230434 - 1158 WP_003898727.1 AI-2E family transporter -
CP465_RS06045 1230546..1230857 - 312 WP_003405820.1 type II toxin-antitoxin system toxin endoribonuclease MazF3 Toxin
CP465_RS06050 1230857..1231093 - 237 WP_003913656.1 hypothetical protein Antitoxin
CP465_RS06055 1231186..1232711 + 1526 Protein_1146 carboxylesterase/lipase family protein -
CP465_RS06060 1232729..1233841 - 1113 WP_003405840.1 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase -
CP465_RS06065 1233851..1234108 - 258 WP_003405844.1 exodeoxyribonuclease VII small subunit -
CP465_RS06070 1234098..1235345 - 1248 WP_003405846.1 exodeoxyribonuclease VII large subunit -
CP465_RS06075 1235342..1235980 - 639 WP_003898730.1 lipid droplet-associated protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase PemK-like

(8-85)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 11061.82 Da        Isoelectric Point: 4.9371

>T83871 WP_003405820.1 NZ_CP023609:c1230857-1230546 [Mycobacterium tuberculosis]
MRPIHIAQLDKARPVLILTREVVRPHLTNVTVAPITTTVRGLATEVPVDAVNGLNQPSVVSCDNIQTIPVCDLGRQIGYL
LASQEPALAEAIGNAFDLDWVVA

Download         Length: 312 bp

>T83871 NZ_CP023609:c1230857-1230546 [Mycobacterium tuberculosis]
ATGCGACCTATCCACATCGCACAGCTTGACAAGGCTAGACCCGTCCTGATCCTTACCCGCGAGGTCGTACGTCCGCACCT
CACGAACGTCACCGTCGCCCCGATCACGACAACCGTGCGTGGGCTAGCCACCGAAGTCCCGGTAGACGCGGTGAACGGGC
TGAATCAACCGTCAGTCGTCAGCTGCGACAACATCCAAACGATCCCGGTGTGTGACCTTGGTCGTCAAATCGGCTACCTG
CTCGCGTCGCAGGAGCCGGCTCTAGCCGAGGCCATCGGCAACGCGTTCGACCTCGATTGGGTGGTGGCATAG

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8608.66 Da        Isoelectric Point: 5.6908

>AT83871 WP_003913656.1 NZ_CP023609:c1231093-1230857 [Mycobacterium tuberculosis]
VSTQIAVRLPDEIVAFIDDEVRGQHARSRAAVVLRALERERRRRLAERDAEILATNTSATGDLDTLAGHCARTALDID

Download         Length: 237 bp

>AT83871 NZ_CP023609:c1231093-1230857 [Mycobacterium tuberculosis]
GTGAGTACACAGATTGCGGTTCGCTTGCCCGATGAGATTGTCGCGTTCATCGACGACGAAGTCCGGGGACAGCATGCCCG
TAGCCGTGCGGCTGTCGTGCTGCGGGCGCTGGAACGGGAACGCCGCCGTCGTCTCGCCGAACGCGACGCGGAGATCCTCG
CCACCAACACGTCAGCCACGGGTGATCTGGATACGCTGGCCGGCCACTGCGCTCGTACCGCCCTCGACATCGACTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4F9D0

Antitoxin

Source ID Structure
AlphaFold DB Q8VK70

References