Detailed information of TA system TA083837

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 2929979..2930693
• Replicon: chromosome
• Accession: NZ_CP023608
• Organism: Mycobacterium tuberculosis strain LE410

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: G0TQK0
• Locus tag: CP464_RS14245
• Protein ID: WP_003413460.1
• Coordinates: 2930253..2930693 (+)
• Length: 147 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: CP464_RS14240
• Protein ID: WP_030030794.1
• Coordinates: 2929979..2930266 (+)
• Length: 96 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
CP464_RS14205	2925401..2925646	+	246	WP_003413429.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	-
CP464_RS14210	2925643..2926047	+	405	WP_003899389.1	type II toxin-antitoxin system VapC family toxin	-
CP464_RS14215	2926264..2926884	+	621	WP_003413441.1	DUF4178 domain-containing protein	-
CP464_RS14220	2926895..2927389	+	495	WP_003413444.1	DUF2617 family protein	-
CP464_RS14225	2927386..2927817	+	432	WP_003899390.1	DUF4247 domain-containing protein	-
CP464_RS14230	2927842..2928300	+	459	WP_003413451.1	DUF350 domain-containing protein	-
CP464_RS14235	2928297..2929868	+	1572	WP_003899392.1	polyamine aminopropyltransferase	-
CP464_RS14240	2929979..2930266	+	288	WP_030030794.1	antitoxin	Antitoxin
CP464_RS14245	2930253..2930693	+	441	WP_003413460.1	type II toxin-antitoxin system VapC family toxin	Toxin
CP464_RS14250	2930714..2931469	-	756	WP_003413464.1	YebC/PmpR family DNA-binding transcriptional regulator	-
CP464_RS14255	2931602..2932198	-	597	WP_003413465.1	pyridoxal 5'-phosphate synthase glutaminase subunit PdxT	-
CP464_RS14260	2932206..2933051	-	846	WP_003413466.1	acyl-CoA thioesterase II	-
CP464_RS14265	2933080..2933979	-	900	WP_003413468.1	pyridoxal 5'-phosphate synthase lyase subunit PdxS	-
CP464_RS14270	2934107..2934781	+	675	WP_003413471.1	pyridoxamine 5'-phosphate oxidase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (1-146)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 147 a.a.
• Molecular weight: 16026.38 Da
• Isoelectric Point: 6.8599

>T83837 WP_003413460.1 NZ_CP023608:2930253-2930693 [Mycobacterium tuberculosis]
MLLCDTNIWLALALSGHVHHRASRAWLDTINAPGVIHFCRATQQSLLRLLTNRTVLGAYGSPPLTNREAWAAYAAFLDDD
RIVLAGAEPDGLEAQWRAFAVRQSPAPKVWMDAYLAAFALTGGFELVTTDTAFTQYGGIELRLLAK

Nucleotide

• Length: 441 bp

>T83837 NZ_CP023608:2930253-2930693 [Mycobacterium tuberculosis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin

Protein

• Length: 96 a.a.
• Molecular weight: 10512.02 Da
• Isoelectric Point: 6.7173

>AT83837 WP_030030794.1 NZ_CP023608:2929979-2930266 [Mycobacterium tuberculosis]
MKTTLDLPDELMRAIKVRAAQQGRKMKDVVTELLRSGLSQTHSGAPIPTPRRVQLPLVHCGDAATREQEMTPERVAAALL
DQEAQWWSGHDDAAL

Nucleotide

• Length: 288 bp

>AT83837 NZ_CP023608:2929979-2930266 [Mycobacterium tuberculosis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGACGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Structures

Toxin

• AlphaFold DB: G0TQK0